CYTOTOXIC EFFECT OF DILLAPIOLE ON HUMAN BREAST CANCER MCF-7 CELLS

• Published in: Malaysian Applied Biology
• Main Author: Amin I.M.; Ruslan N.B.; Zulkipli Z.A.; Zakaria N.A.; Jalil M.T.M.; Aris F.
• Format: Article
• Language: English
• Published: Malaysian Society of Applied Biology 2022
• Online Access: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85140959060&doi=10.55230%2fmabjournal.v51i4.08&partnerID=40&md5=e79587122b238d039e18b94484af0d32

Plant secondary metabolites and their derivatives play a significant role in anticancer drug therapy since they are effective against specific characteristics while reducing side effects. Dillapiole is a phenylpropanoid that holds several bioactivities like anti-fungal, anti-inflammatory, anti-leishmanial, and anti-cancer. This study aims to investigate the possible cytotoxic effect of dillapiole on human breast cancer, MCF-7 cells. Cells were cultured in complete RPMI media and incubated at standard culture conditions. After the cells reached 80% confluency, cells were treated with various concentrations (ranging from 0 µM to 150 µM) of dillapiole and tamoxifen as a positive control. Cells were later incubated at 48 and 72 h. Using WST-1 assay, the cytotoxic effect was determined for both incubation times. Results show tamoxifen inhibited the MCF-7 cells with the IC50 at 75.9 µM and 39.8 µM for 48 and 72 h respectively. Parallel with the positive control results, there was a significant cytotoxic effect of dillapiole against MCF-7 cells at 48-and 72-hr incubation with the IC50 at 92.1 µM and 63.1 µM respectively. Based on these results, dillapiole was cytotoxic against MCF-7 cells and its cytotoxic activity was both in a time and dose-dependent manner (<0.05). The morphological analysis supported the WST-1 assay. Our preliminary finding is in agreement with other previous studies, suggesting that dillapiole appears to be a promising anti-cancer agent and opens a wider possibility of downstream analysis on its underlying cytotoxicity mechanism. © 2022, Malaysian Society of Applied Biology. All rights reserved.