ANTIBACTERIAL AND ANTIBIOFILM ACTIVITIES OF Swietenia macrophylla King ETHANOLIC EXTRACT AGAINST FOODBORNE PATHOGENS

Swietenia macrophylla is known to possess several medicinal uses, however, its antibacterial and antibiofilm activities against foodborne pathogens remain not well investigated. The present work was performed to examine the phytochemical compounds, antibacterial and antibiofilm activities of S. macr...

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Published in:Malaysian Applied Biology
Main Author: Man C.A.I.C.; Razak W.R.W.A.; Yahya M.F.Z.R.
Format: Article
Language:English
Published: Malaysian Society of Applied Biology 2022
Online Access:https://www.scopus.com/inward/record.uri?eid=2-s2.0-85140958445&doi=10.55230%2fmabjournal.v51i4.10&partnerID=40&md5=83f0197de13276fa6dd6053988054622
id 2-s2.0-85140958445
spelling 2-s2.0-85140958445
Man C.A.I.C.; Razak W.R.W.A.; Yahya M.F.Z.R.
ANTIBACTERIAL AND ANTIBIOFILM ACTIVITIES OF Swietenia macrophylla King ETHANOLIC EXTRACT AGAINST FOODBORNE PATHOGENS
2022
Malaysian Applied Biology
51
4
10.55230/mabjournal.v51i4.10
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85140958445&doi=10.55230%2fmabjournal.v51i4.10&partnerID=40&md5=83f0197de13276fa6dd6053988054622
Swietenia macrophylla is known to possess several medicinal uses, however, its antibacterial and antibiofilm activities against foodborne pathogens remain not well investigated. The present work was performed to examine the phytochemical compounds, antibacterial and antibiofilm activities of S. macrophylla ethanolic extract (SMEE) against four foodborne pathogens namely, Salmonella typhimurium ATCC 14028, Escherichia coli ATCC 25922, Shigella sonnei ATCC 33862 and Pseudomonas aeruginosa ATCC 10145. The phytochemical analysis of SMEE was performed using gas chromatography-mass spectrometry while the antibacterial activities of SMEE were determined by minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) assays. On the other hand, the antibiofilm and time-killing activities of SMME were evaluated using a crystal violet assay. The result demonstrated that SMEE contained major phytochemical compounds such as olean-12-ene (27.37%), resorcinol (16.45%), 24-noroleana-3,12-diene (13.4%), and germanicol (11.50%). The MIC values of SMEE ranged from 31.25 to 500 µg/mL, while all the MBC values were found to be greater than 1000 µg/mL. At the 12 h exposure to SMEE, all the biofilms were inhibited by 50% except E. coli. Biofilm inhibitory concentration (BIC50) values of SMEE ranged between 5.19 and 42.47 µg/mL. In conclusion, S. macrophylla is a promising source of natural antibacterial and antibiofilm agents to treat foodborne diseases. © 2022, Malaysian Society of Applied Biology. All rights reserved.
Malaysian Society of Applied Biology
1268643
English
Article
All Open Access; Bronze Open Access
author Man C.A.I.C.; Razak W.R.W.A.; Yahya M.F.Z.R.
spellingShingle Man C.A.I.C.; Razak W.R.W.A.; Yahya M.F.Z.R.
ANTIBACTERIAL AND ANTIBIOFILM ACTIVITIES OF Swietenia macrophylla King ETHANOLIC EXTRACT AGAINST FOODBORNE PATHOGENS
author_facet Man C.A.I.C.; Razak W.R.W.A.; Yahya M.F.Z.R.
author_sort Man C.A.I.C.; Razak W.R.W.A.; Yahya M.F.Z.R.
title ANTIBACTERIAL AND ANTIBIOFILM ACTIVITIES OF Swietenia macrophylla King ETHANOLIC EXTRACT AGAINST FOODBORNE PATHOGENS
title_short ANTIBACTERIAL AND ANTIBIOFILM ACTIVITIES OF Swietenia macrophylla King ETHANOLIC EXTRACT AGAINST FOODBORNE PATHOGENS
title_full ANTIBACTERIAL AND ANTIBIOFILM ACTIVITIES OF Swietenia macrophylla King ETHANOLIC EXTRACT AGAINST FOODBORNE PATHOGENS
title_fullStr ANTIBACTERIAL AND ANTIBIOFILM ACTIVITIES OF Swietenia macrophylla King ETHANOLIC EXTRACT AGAINST FOODBORNE PATHOGENS
title_full_unstemmed ANTIBACTERIAL AND ANTIBIOFILM ACTIVITIES OF Swietenia macrophylla King ETHANOLIC EXTRACT AGAINST FOODBORNE PATHOGENS
title_sort ANTIBACTERIAL AND ANTIBIOFILM ACTIVITIES OF Swietenia macrophylla King ETHANOLIC EXTRACT AGAINST FOODBORNE PATHOGENS
publishDate 2022
container_title Malaysian Applied Biology
container_volume 51
container_issue 4
doi_str_mv 10.55230/mabjournal.v51i4.10
url https://www.scopus.com/inward/record.uri?eid=2-s2.0-85140958445&doi=10.55230%2fmabjournal.v51i4.10&partnerID=40&md5=83f0197de13276fa6dd6053988054622
description Swietenia macrophylla is known to possess several medicinal uses, however, its antibacterial and antibiofilm activities against foodborne pathogens remain not well investigated. The present work was performed to examine the phytochemical compounds, antibacterial and antibiofilm activities of S. macrophylla ethanolic extract (SMEE) against four foodborne pathogens namely, Salmonella typhimurium ATCC 14028, Escherichia coli ATCC 25922, Shigella sonnei ATCC 33862 and Pseudomonas aeruginosa ATCC 10145. The phytochemical analysis of SMEE was performed using gas chromatography-mass spectrometry while the antibacterial activities of SMEE were determined by minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) assays. On the other hand, the antibiofilm and time-killing activities of SMME were evaluated using a crystal violet assay. The result demonstrated that SMEE contained major phytochemical compounds such as olean-12-ene (27.37%), resorcinol (16.45%), 24-noroleana-3,12-diene (13.4%), and germanicol (11.50%). The MIC values of SMEE ranged from 31.25 to 500 µg/mL, while all the MBC values were found to be greater than 1000 µg/mL. At the 12 h exposure to SMEE, all the biofilms were inhibited by 50% except E. coli. Biofilm inhibitory concentration (BIC50) values of SMEE ranged between 5.19 and 42.47 µg/mL. In conclusion, S. macrophylla is a promising source of natural antibacterial and antibiofilm agents to treat foodborne diseases. © 2022, Malaysian Society of Applied Biology. All rights reserved.
publisher Malaysian Society of Applied Biology
issn 1268643
language English
format Article
accesstype All Open Access; Bronze Open Access
record_format scopus
collection Scopus
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