| Summary: | Background: Dryobalanops rappa is a plant species belonging to the family of Diptero-carpaceae. Nevertheless, the active compounds present in D. rappa have never been investigated. Objective: The aim of this research is to isolate and characterize compounds from Dryobalanops rappa and to study its bioactivity against human MCF-7 breast cancer and A549 lung cancer cell lines and several bacterial strains. Methods: The isolation step was carried out using a combination of chromatographic techniques. The structure of the isolated compounds was elucidated mainly using NMR spectroscopy. The cyto-toxic activity of isolated compounds was determined with MTT assay, and the antimicrobial was screened using a modified resazurin microtiter-plate assay. Results: Isolation and purification of methanolic extract of D. rappa stem bark yielded 14 known oligomeric resveratrol types of compounds (1-14). Results showed that isolated ampelopsin E (5) and vaticanol C (14) displayed moderate activity against human MCF-7 breast cancer and A549 lung cancer cell lines with IC50 values 14.3 and 10.7 μg/mL, respectively. Interestingly, acetate derivative of isolated laevifonol (2) and ampelopsin F (3) was found to possess potent activities towards MCF-7 cancer cell line with IC50 values 2.8 and 3.3 μg/mL, respectively, in comparison to the parental compounds that demonstrated weak activities (IC50 > 50 μg/mL). For the antibacterial assay, compounds 10 and 12 showed moderate activities towards Gram-positive bacterial strains (MIC ≤ 50 μM). Conclusion: 14 known oligomeric resveratrol types of compounds have been isolated. The activity of 14 against A549 cell line, 5 against MCF-7 cell line, and 10 and 12 against Gram-positive bacterial strains were the most promising results of this study. While the strong cytotoxicity of acetate derivative (2 and 3) against MCF-7 cell line has revealed the potential of resveratrol oligomers to be used as a template for designing new anticancer drugs. © 2022 Bentham Science Publishers.
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