Effect ethyl acetate extract of Lasiodiplodia pseudotheobromae Industrial Biotechnology Research Laboratory OS-64 on growth and cell morphology of a foodborne bacterium, Bacillus cereus
Background: A foodborne bacterium, Bacillus cereus is one of the common food contaminants that could cause food poising with several sickness symptoms to humans. Objective: This study was undertaken to evaluate the effect of ethyl acetate extract of endophytic fungus, Lasiodiplodia pseudotheobromae...
Published in: | Thai Journal of Pharmaceutical Sciences |
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Faculty of Pharmaceutical Sciences, Chulalongkorn University
2021
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2-s2.0-85123522520 Jalil M.T.M.; Ibrahim D. Effect ethyl acetate extract of Lasiodiplodia pseudotheobromae Industrial Biotechnology Research Laboratory OS-64 on growth and cell morphology of a foodborne bacterium, Bacillus cereus 2021 Thai Journal of Pharmaceutical Sciences 45 6 10.56808/3027-7922.2536 https://www.scopus.com/inward/record.uri?eid=2-s2.0-85123522520&doi=10.56808%2f3027-7922.2536&partnerID=40&md5=aab1bba75db835c36d1ae4ab65ade6db Background: A foodborne bacterium, Bacillus cereus is one of the common food contaminants that could cause food poising with several sickness symptoms to humans. Objective: This study was undertaken to evaluate the effect of ethyl acetate extract of endophytic fungus, Lasiodiplodia pseudotheobromae Industrial Biotechnology Research Laboratory (IBRL) OS-64 towards a test bacterium, B. cereus with emphasis on bacterial growth reduction and cell morphological changes. Materials and Methods: The ethyl acetate (fermentative broth) and methanolic (fungal biomass) extracts were screened for their antibacterial activity against test bacteria via agar disc diffusion assay. Results: The results demonstrated that there is a significant antibacterial activity of the ethyl acetate extract with a diameter zone of inhibition in the value of 22.0 ± 0.6 mm. However, no antibacterial activity was observed from the methanolic extract. In broth microdilution assay, the minimum inhibitory concentration and minimum bactericidal concentration values of the extract were 250 and 500 µg/mL, respectively. The results revealed that the extract exhibited a bactericidal effect on the test bacterium. A time-kill study was then performed to determine the efficacy of the extract and the results showed that the antibacterial activity of the extract was dose and time-dependent. The morphological changes of the bacterial cells exposed to the extract were observed under a scanning electron microscope and transmission electron microscope. The results revealed the abnormalities of the treated bacterial cells compared to the control and thus led to ultimate death beyond repair. Conclusion: The present study suggested the potency of the ethyl acetate extract from an endophytic fungus, L. pseudotheobromae IBRL OS-64 could be further used as an antibacterial agent. © 2021, Faculty of Pharmaceutical Sciences, Chulalongkorn University. All rights reserved. Faculty of Pharmaceutical Sciences, Chulalongkorn University 19054637 English Article All Open Access; Bronze Open Access |
author |
Jalil M.T.M.; Ibrahim D. |
spellingShingle |
Jalil M.T.M.; Ibrahim D. Effect ethyl acetate extract of Lasiodiplodia pseudotheobromae Industrial Biotechnology Research Laboratory OS-64 on growth and cell morphology of a foodborne bacterium, Bacillus cereus |
author_facet |
Jalil M.T.M.; Ibrahim D. |
author_sort |
Jalil M.T.M.; Ibrahim D. |
title |
Effect ethyl acetate extract of Lasiodiplodia pseudotheobromae Industrial Biotechnology Research Laboratory OS-64 on growth and cell morphology of a foodborne bacterium, Bacillus cereus |
title_short |
Effect ethyl acetate extract of Lasiodiplodia pseudotheobromae Industrial Biotechnology Research Laboratory OS-64 on growth and cell morphology of a foodborne bacterium, Bacillus cereus |
title_full |
Effect ethyl acetate extract of Lasiodiplodia pseudotheobromae Industrial Biotechnology Research Laboratory OS-64 on growth and cell morphology of a foodborne bacterium, Bacillus cereus |
title_fullStr |
Effect ethyl acetate extract of Lasiodiplodia pseudotheobromae Industrial Biotechnology Research Laboratory OS-64 on growth and cell morphology of a foodborne bacterium, Bacillus cereus |
title_full_unstemmed |
Effect ethyl acetate extract of Lasiodiplodia pseudotheobromae Industrial Biotechnology Research Laboratory OS-64 on growth and cell morphology of a foodborne bacterium, Bacillus cereus |
title_sort |
Effect ethyl acetate extract of Lasiodiplodia pseudotheobromae Industrial Biotechnology Research Laboratory OS-64 on growth and cell morphology of a foodborne bacterium, Bacillus cereus |
publishDate |
2021 |
container_title |
Thai Journal of Pharmaceutical Sciences |
container_volume |
45 |
container_issue |
6 |
doi_str_mv |
10.56808/3027-7922.2536 |
url |
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85123522520&doi=10.56808%2f3027-7922.2536&partnerID=40&md5=aab1bba75db835c36d1ae4ab65ade6db |
description |
Background: A foodborne bacterium, Bacillus cereus is one of the common food contaminants that could cause food poising with several sickness symptoms to humans. Objective: This study was undertaken to evaluate the effect of ethyl acetate extract of endophytic fungus, Lasiodiplodia pseudotheobromae Industrial Biotechnology Research Laboratory (IBRL) OS-64 towards a test bacterium, B. cereus with emphasis on bacterial growth reduction and cell morphological changes. Materials and Methods: The ethyl acetate (fermentative broth) and methanolic (fungal biomass) extracts were screened for their antibacterial activity against test bacteria via agar disc diffusion assay. Results: The results demonstrated that there is a significant antibacterial activity of the ethyl acetate extract with a diameter zone of inhibition in the value of 22.0 ± 0.6 mm. However, no antibacterial activity was observed from the methanolic extract. In broth microdilution assay, the minimum inhibitory concentration and minimum bactericidal concentration values of the extract were 250 and 500 µg/mL, respectively. The results revealed that the extract exhibited a bactericidal effect on the test bacterium. A time-kill study was then performed to determine the efficacy of the extract and the results showed that the antibacterial activity of the extract was dose and time-dependent. The morphological changes of the bacterial cells exposed to the extract were observed under a scanning electron microscope and transmission electron microscope. The results revealed the abnormalities of the treated bacterial cells compared to the control and thus led to ultimate death beyond repair. Conclusion: The present study suggested the potency of the ethyl acetate extract from an endophytic fungus, L. pseudotheobromae IBRL OS-64 could be further used as an antibacterial agent. © 2021, Faculty of Pharmaceutical Sciences, Chulalongkorn University. All rights reserved. |
publisher |
Faculty of Pharmaceutical Sciences, Chulalongkorn University |
issn |
19054637 |
language |
English |
format |
Article |
accesstype |
All Open Access; Bronze Open Access |
record_format |
scopus |
collection |
Scopus |
_version_ |
1814778506144382976 |