The High Occurrence of Multidrug-Resistant Salmonella spp. Isolated from Raw Chicken Meat and Contact Surfaces at Wet Market in Malaysia

Post-slaughter processing steps during poultry meat production contribute to Salmonella contamination and may transmit multidrug-resistant Salmonella to chicken meats and contact surfaces. This study was undertaken to identify Salmonella spp. strains isolated from chicken meats and their contact sur...

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Published in:Sains Malaysiana
Main Author: Sukri A.; Zulfakar S.S.; Taib I.S.M.; Omar N.F.; Zin N.M.
Format: Article
Language:English
Published: Penerbit Universiti Kebangsaan Malaysia 2021
Online Access:https://www.scopus.com/inward/record.uri?eid=2-s2.0-85122271290&doi=10.17576%2fjsm-2021-5012-25&partnerID=40&md5=4a9259ee450e2af5336c632aa10f8b37
id 2-s2.0-85122271290
spelling 2-s2.0-85122271290
Sukri A.; Zulfakar S.S.; Taib I.S.M.; Omar N.F.; Zin N.M.
The High Occurrence of Multidrug-Resistant Salmonella spp. Isolated from Raw Chicken Meat and Contact Surfaces at Wet Market in Malaysia
2021
Sains Malaysiana
50
12
10.17576/jsm-2021-5012-25
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85122271290&doi=10.17576%2fjsm-2021-5012-25&partnerID=40&md5=4a9259ee450e2af5336c632aa10f8b37
Post-slaughter processing steps during poultry meat production contribute to Salmonella contamination and may transmit multidrug-resistant Salmonella to chicken meats and contact surfaces. This study was undertaken to identify Salmonella spp. strains isolated from chicken meats and their contact surfaces, and to determine their antibiotic resistance profiles. A total of 20 samples were collected from raw chicken meat and its contact surfaces from a shop in Pasar Chow Kit, Kuala Lumpur. Identification of presumptive Salmonella colonies was conducted by using biochemical tests and multiplex polymerase chain reaction (PCR). Antibiotic susceptibility test was conducted by using Kirby-Bauer disk diffusion method against 11 antibiotics. Salmonella enterica Group I was only detected in seven samples. The antibiogram testing showed that all the seven Salmonella isolates (100%) were multidrug-resistant and all isolates were resistant to penicillin, chloramphenicol, erythromycin, and tetracycline. Resistance towards trimethoprim-sulfamethoxazole, amoxicillin and ampicillin was observed in four isolates. However, sensitivity was recorded for cephalexin, cefoxitin, cefotaxime, and ceftriaxone in six isolates. The highest multiple antibiotic resistance (MAR) index of 1.00 (resistance to all eleven antibiotics tested) was observed in one isolate (P2) while the lowest MAR index of 0.36 (resistance to four antibiotics tested) was observed in three isolates (CB, M2 and M3). Our results demonstrated that raw chicken and meat contact surface were a source of multidrug-resistant Salmonella and can contribute to significant health concern in Malaysia. Stringent hygienic procedure during chicken meat processing should be therefore be practised. © 2021 Penerbit Universiti Kebangsaan Malaysia. All rights reserved.
Penerbit Universiti Kebangsaan Malaysia
1266039
English
Article
All Open Access; Gold Open Access
author Sukri A.; Zulfakar S.S.; Taib I.S.M.; Omar N.F.; Zin N.M.
spellingShingle Sukri A.; Zulfakar S.S.; Taib I.S.M.; Omar N.F.; Zin N.M.
The High Occurrence of Multidrug-Resistant Salmonella spp. Isolated from Raw Chicken Meat and Contact Surfaces at Wet Market in Malaysia
author_facet Sukri A.; Zulfakar S.S.; Taib I.S.M.; Omar N.F.; Zin N.M.
author_sort Sukri A.; Zulfakar S.S.; Taib I.S.M.; Omar N.F.; Zin N.M.
title The High Occurrence of Multidrug-Resistant Salmonella spp. Isolated from Raw Chicken Meat and Contact Surfaces at Wet Market in Malaysia
title_short The High Occurrence of Multidrug-Resistant Salmonella spp. Isolated from Raw Chicken Meat and Contact Surfaces at Wet Market in Malaysia
title_full The High Occurrence of Multidrug-Resistant Salmonella spp. Isolated from Raw Chicken Meat and Contact Surfaces at Wet Market in Malaysia
title_fullStr The High Occurrence of Multidrug-Resistant Salmonella spp. Isolated from Raw Chicken Meat and Contact Surfaces at Wet Market in Malaysia
title_full_unstemmed The High Occurrence of Multidrug-Resistant Salmonella spp. Isolated from Raw Chicken Meat and Contact Surfaces at Wet Market in Malaysia
title_sort The High Occurrence of Multidrug-Resistant Salmonella spp. Isolated from Raw Chicken Meat and Contact Surfaces at Wet Market in Malaysia
publishDate 2021
container_title Sains Malaysiana
container_volume 50
container_issue 12
doi_str_mv 10.17576/jsm-2021-5012-25
url https://www.scopus.com/inward/record.uri?eid=2-s2.0-85122271290&doi=10.17576%2fjsm-2021-5012-25&partnerID=40&md5=4a9259ee450e2af5336c632aa10f8b37
description Post-slaughter processing steps during poultry meat production contribute to Salmonella contamination and may transmit multidrug-resistant Salmonella to chicken meats and contact surfaces. This study was undertaken to identify Salmonella spp. strains isolated from chicken meats and their contact surfaces, and to determine their antibiotic resistance profiles. A total of 20 samples were collected from raw chicken meat and its contact surfaces from a shop in Pasar Chow Kit, Kuala Lumpur. Identification of presumptive Salmonella colonies was conducted by using biochemical tests and multiplex polymerase chain reaction (PCR). Antibiotic susceptibility test was conducted by using Kirby-Bauer disk diffusion method against 11 antibiotics. Salmonella enterica Group I was only detected in seven samples. The antibiogram testing showed that all the seven Salmonella isolates (100%) were multidrug-resistant and all isolates were resistant to penicillin, chloramphenicol, erythromycin, and tetracycline. Resistance towards trimethoprim-sulfamethoxazole, amoxicillin and ampicillin was observed in four isolates. However, sensitivity was recorded for cephalexin, cefoxitin, cefotaxime, and ceftriaxone in six isolates. The highest multiple antibiotic resistance (MAR) index of 1.00 (resistance to all eleven antibiotics tested) was observed in one isolate (P2) while the lowest MAR index of 0.36 (resistance to four antibiotics tested) was observed in three isolates (CB, M2 and M3). Our results demonstrated that raw chicken and meat contact surface were a source of multidrug-resistant Salmonella and can contribute to significant health concern in Malaysia. Stringent hygienic procedure during chicken meat processing should be therefore be practised. © 2021 Penerbit Universiti Kebangsaan Malaysia. All rights reserved.
publisher Penerbit Universiti Kebangsaan Malaysia
issn 1266039
language English
format Article
accesstype All Open Access; Gold Open Access
record_format scopus
collection Scopus
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