Ficus deltoidea ameliorates biochemical, hormonal, and histomorphometric changes in letrozole-induced polycystic ovarian syndrome rats

Background: Insulin resistance and hormonal imbalances are key features in the pathophysiology of polycystic ovarian syndrome (PCOS). We have previously shown that Ficus deltoidea var. deltoidea Jack (Moraceae) can improve insulin sensitivity and hormonal profile in PCOS female rats. However, biolog...

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Published in:BMC Complementary Medicine and Therapies
Main Author: Haslan M.A.; Samsulrizal N.; Hashim N.; Zin N.S.N.M.; Shirazi F.H.; Goh Y.M.
Format: Article
Language:English
Published: BioMed Central Ltd 2021
Online Access:https://www.scopus.com/inward/record.uri?eid=2-s2.0-85120170860&doi=10.1186%2fs12906-021-03452-6&partnerID=40&md5=f2df520f12d81b21df0bff8d8e85a1ed
id 2-s2.0-85120170860
spelling 2-s2.0-85120170860
Haslan M.A.; Samsulrizal N.; Hashim N.; Zin N.S.N.M.; Shirazi F.H.; Goh Y.M.
Ficus deltoidea ameliorates biochemical, hormonal, and histomorphometric changes in letrozole-induced polycystic ovarian syndrome rats
2021
BMC Complementary Medicine and Therapies
21
1
10.1186/s12906-021-03452-6
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85120170860&doi=10.1186%2fs12906-021-03452-6&partnerID=40&md5=f2df520f12d81b21df0bff8d8e85a1ed
Background: Insulin resistance and hormonal imbalances are key features in the pathophysiology of polycystic ovarian syndrome (PCOS). We have previously shown that Ficus deltoidea var. deltoidea Jack (Moraceae) can improve insulin sensitivity and hormonal profile in PCOS female rats. However, biological characteristics underpinning the therapeutic effects of F. deltoidea for treating PCOS remain to be clarified. This study aims to investigate the biochemical, hormonal, and histomorphometric changes in letrozole (LTZ)-induced PCOS female rats following treatment with F. deltoidea. Methods: PCOS was induced in rats except for normal control by administering LTZ at 1 mg/kg/day for 21 days. Methanolic extract of F. deltoidea leaf was then orally administered to the PCOS rats at the dose of 250, 500, or 1000 mg/kg/day, respectively for 15 consecutive days. Lipid profile was measured enzymatically in serum. The circulating concentrations of reproductive hormone and antioxidant enzymes were determined by ELISA assays. Ovarian and uterus histomorphometric changes were further observed by hematoxylin and eosin (H&E) staining. Results: The results showed that treatment with F. deltoidea at the dose of 500 and 1000 mg/kg/day reduced insulin resistance, obesity indices, total cholesterol, triglycerides, low-density lipoprotein cholesterol (LDL), malondialdehyde (MDA), testosterone, luteinizing hormone (LH), and follicle-stimulating hormone (FSH) to near-normal levels in PCOS rats. The levels of high-density lipoprotein cholesterol (HDL), estrogen, and superoxide dismutase (SOD) are also similar to those observed in normal control rats. Histomorphometric measurements confirmed that F. deltoidea increased the corpus luteum number and the endometrial thickness. Conclusions: F. deltoidea can reverse PCOS symptoms in female rats by improving insulin sensitivity, antioxidant activities, hormonal imbalance, and histological changes. These findings suggest the potential use of F. deltoidea as an adjuvant agent in the treatment program of PCOS. © 2021, The Author(s).
BioMed Central Ltd
26627671
English
Article
All Open Access; Gold Open Access
author Haslan M.A.; Samsulrizal N.; Hashim N.; Zin N.S.N.M.; Shirazi F.H.; Goh Y.M.
spellingShingle Haslan M.A.; Samsulrizal N.; Hashim N.; Zin N.S.N.M.; Shirazi F.H.; Goh Y.M.
Ficus deltoidea ameliorates biochemical, hormonal, and histomorphometric changes in letrozole-induced polycystic ovarian syndrome rats
author_facet Haslan M.A.; Samsulrizal N.; Hashim N.; Zin N.S.N.M.; Shirazi F.H.; Goh Y.M.
author_sort Haslan M.A.; Samsulrizal N.; Hashim N.; Zin N.S.N.M.; Shirazi F.H.; Goh Y.M.
title Ficus deltoidea ameliorates biochemical, hormonal, and histomorphometric changes in letrozole-induced polycystic ovarian syndrome rats
title_short Ficus deltoidea ameliorates biochemical, hormonal, and histomorphometric changes in letrozole-induced polycystic ovarian syndrome rats
title_full Ficus deltoidea ameliorates biochemical, hormonal, and histomorphometric changes in letrozole-induced polycystic ovarian syndrome rats
title_fullStr Ficus deltoidea ameliorates biochemical, hormonal, and histomorphometric changes in letrozole-induced polycystic ovarian syndrome rats
title_full_unstemmed Ficus deltoidea ameliorates biochemical, hormonal, and histomorphometric changes in letrozole-induced polycystic ovarian syndrome rats
title_sort Ficus deltoidea ameliorates biochemical, hormonal, and histomorphometric changes in letrozole-induced polycystic ovarian syndrome rats
publishDate 2021
container_title BMC Complementary Medicine and Therapies
container_volume 21
container_issue 1
doi_str_mv 10.1186/s12906-021-03452-6
url https://www.scopus.com/inward/record.uri?eid=2-s2.0-85120170860&doi=10.1186%2fs12906-021-03452-6&partnerID=40&md5=f2df520f12d81b21df0bff8d8e85a1ed
description Background: Insulin resistance and hormonal imbalances are key features in the pathophysiology of polycystic ovarian syndrome (PCOS). We have previously shown that Ficus deltoidea var. deltoidea Jack (Moraceae) can improve insulin sensitivity and hormonal profile in PCOS female rats. However, biological characteristics underpinning the therapeutic effects of F. deltoidea for treating PCOS remain to be clarified. This study aims to investigate the biochemical, hormonal, and histomorphometric changes in letrozole (LTZ)-induced PCOS female rats following treatment with F. deltoidea. Methods: PCOS was induced in rats except for normal control by administering LTZ at 1 mg/kg/day for 21 days. Methanolic extract of F. deltoidea leaf was then orally administered to the PCOS rats at the dose of 250, 500, or 1000 mg/kg/day, respectively for 15 consecutive days. Lipid profile was measured enzymatically in serum. The circulating concentrations of reproductive hormone and antioxidant enzymes were determined by ELISA assays. Ovarian and uterus histomorphometric changes were further observed by hematoxylin and eosin (H&E) staining. Results: The results showed that treatment with F. deltoidea at the dose of 500 and 1000 mg/kg/day reduced insulin resistance, obesity indices, total cholesterol, triglycerides, low-density lipoprotein cholesterol (LDL), malondialdehyde (MDA), testosterone, luteinizing hormone (LH), and follicle-stimulating hormone (FSH) to near-normal levels in PCOS rats. The levels of high-density lipoprotein cholesterol (HDL), estrogen, and superoxide dismutase (SOD) are also similar to those observed in normal control rats. Histomorphometric measurements confirmed that F. deltoidea increased the corpus luteum number and the endometrial thickness. Conclusions: F. deltoidea can reverse PCOS symptoms in female rats by improving insulin sensitivity, antioxidant activities, hormonal imbalance, and histological changes. These findings suggest the potential use of F. deltoidea as an adjuvant agent in the treatment program of PCOS. © 2021, The Author(s).
publisher BioMed Central Ltd
issn 26627671
language English
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accesstype All Open Access; Gold Open Access
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