Sperm vitality and necrozoospermia: diagnosis, management, and results of a global survey of clinical practice

Sperm vitality and necrozoospermia: diagnosis, management, and results of a global survey of clinical practice

Sperm vitality testing is a basic semen examination that has been described in the World Health Organization (WHO) Laboratory Manual for the Examination and Processing of Human Semen from its primary edition, 40 years ago. Several methods can be used to test sperm vitality, such as the eosin-nigrosi...

Full description

Bibliographic Details
Published in:World Journal of Men's Health
Main Author: Agarwal A.; Sharma R.K.; Gupta S.; Boitrelle F.; Finelli R.; Parekh N.; Durairajanayagam D.; Saleh R.; Arafa M.; Cho C.L.; Farkouh A.; Rambhatla A.; Henkel R.; Vogiatzi P.; Tadros N.; Kavoussi P.; Ko E.; Leisegang K.; Kandil H.; Palani A.; Salvio G.; Mostafa T.; Rajmil O.; Banihani S.A.; Schon S.; Le T.V.; Birowo P.; Çeker G.; Alvarez J.; Molina J.M.C.; Ho C.C.K.; Calogero A.E.; Khalafalla K.; Duran M.B.; Kuroda S.; Colpi G.M.; Zini A.; Anagnostopoulou C.; Pescatori E.; Chung E.; Caroppo E.; Dimitriadis F.; Pinggera G.-M.; Busetto G.M.; Balercia G.; Elbardisi H.; Taniguchi H.; Park H.J.; Rosas I.M.; de la Rosette J.; Ramsay J.; Bowa K.; Simopoulou M.; Rodriguez M.G.; Sabbaghian M.; Martinez M.; Gilani M.A.S.; Al-Marhoon M.S.; Kosgi R.; Cannarella R.; Micic S.; Fukuhara S.; Parekattil S.; Jindal S.; Abdel-Meguid T.A.-A.; Morimoto Y.; Shah R.
Format: Review
Language:English
Published: Korean Society for Sexual Medicine and Andrology 2022
Online Access:https://www.scopus.com/inward/record.uri?eid=2-s2.0-85118736952&doi=10.5534%2fwjmh.210149&partnerID=40&md5=776ce144f9bcad4451df84b64415ccd7
id 2-s2.0-85118736952
spelling 2-s2.0-85118736952
Agarwal A.; Sharma R.K.; Gupta S.; Boitrelle F.; Finelli R.; Parekh N.; Durairajanayagam D.; Saleh R.; Arafa M.; Cho C.L.; Farkouh A.; Rambhatla A.; Henkel R.; Vogiatzi P.; Tadros N.; Kavoussi P.; Ko E.; Leisegang K.; Kandil H.; Palani A.; Salvio G.; Mostafa T.; Rajmil O.; Banihani S.A.; Schon S.; Le T.V.; Birowo P.; Çeker G.; Alvarez J.; Molina J.M.C.; Ho C.C.K.; Calogero A.E.; Khalafalla K.; Duran M.B.; Kuroda S.; Colpi G.M.; Zini A.; Anagnostopoulou C.; Pescatori E.; Chung E.; Caroppo E.; Dimitriadis F.; Pinggera G.-M.; Busetto G.M.; Balercia G.; Elbardisi H.; Taniguchi H.; Park H.J.; Rosas I.M.; de la Rosette J.; Ramsay J.; Bowa K.; Simopoulou M.; Rodriguez M.G.; Sabbaghian M.; Martinez M.; Gilani M.A.S.; Al-Marhoon M.S.; Kosgi R.; Cannarella R.; Micic S.; Fukuhara S.; Parekattil S.; Jindal S.; Abdel-Meguid T.A.-A.; Morimoto Y.; Shah R.
Sperm vitality and necrozoospermia: diagnosis, management, and results of a global survey of clinical practice
2022
World Journal of Men's Health
40
2
10.5534/wjmh.210149
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85118736952&doi=10.5534%2fwjmh.210149&partnerID=40&md5=776ce144f9bcad4451df84b64415ccd7
Sperm vitality testing is a basic semen examination that has been described in the World Health Organization (WHO) Laboratory Manual for the Examination and Processing of Human Semen from its primary edition, 40 years ago. Several methods can be used to test sperm vitality, such as the eosin-nigrosin (E-N) stain or the hypoosmotic swelling (HOS) test. In the 6th (2021) edition of the WHO Laboratory Manual, sperm vitality assessment is mainly recommended if the total motility is less than 40%. Hence, a motile spermatozoon is considered alive, however, in certain conditions an immotile spermatozoon can also be alive. Therefore, the differentiation between asthenozoospermia (pathological decrease in sperm motility) and necrozoospermia (pathological decrease in sperm vitality) is important in directing further investigation and management of infertile patients. The causes leading to necrozoospermia are diverse and can either be local or general, testicular or extra-testicular. The andrological management of necrozoospermia depends on its etiology. However, there is no standardized treatment available presently and practice varies among clinicians. In this study, we report the results of a global survey to understand current practices regarding the physician order of sperm vitality tests as well as the management practices for necrozoospermia. Laboratory and clinical scenarios are presented to guide the reader in the management of necrozoospermia with the overall objective of establishing a benchmark ranging from the diagnosis of necrozoospermia by sperm vitality testing to its clinical management. Copyright © 2021 Korean Society for Sexual Medicine and Andrology
Korean Society for Sexual Medicine and Andrology
22874208
English
Review
All Open Access; Gold Open Access
author Agarwal A.; Sharma R.K.; Gupta S.; Boitrelle F.; Finelli R.; Parekh N.; Durairajanayagam D.; Saleh R.; Arafa M.; Cho C.L.; Farkouh A.; Rambhatla A.; Henkel R.; Vogiatzi P.; Tadros N.; Kavoussi P.; Ko E.; Leisegang K.; Kandil H.; Palani A.; Salvio G.; Mostafa T.; Rajmil O.; Banihani S.A.; Schon S.; Le T.V.; Birowo P.; Çeker G.; Alvarez J.; Molina J.M.C.; Ho C.C.K.; Calogero A.E.; Khalafalla K.; Duran M.B.; Kuroda S.; Colpi G.M.; Zini A.; Anagnostopoulou C.; Pescatori E.; Chung E.; Caroppo E.; Dimitriadis F.; Pinggera G.-M.; Busetto G.M.; Balercia G.; Elbardisi H.; Taniguchi H.; Park H.J.; Rosas I.M.; de la Rosette J.; Ramsay J.; Bowa K.; Simopoulou M.; Rodriguez M.G.; Sabbaghian M.; Martinez M.; Gilani M.A.S.; Al-Marhoon M.S.; Kosgi R.; Cannarella R.; Micic S.; Fukuhara S.; Parekattil S.; Jindal S.; Abdel-Meguid T.A.-A.; Morimoto Y.; Shah R.
spellingShingle Agarwal A.; Sharma R.K.; Gupta S.; Boitrelle F.; Finelli R.; Parekh N.; Durairajanayagam D.; Saleh R.; Arafa M.; Cho C.L.; Farkouh A.; Rambhatla A.; Henkel R.; Vogiatzi P.; Tadros N.; Kavoussi P.; Ko E.; Leisegang K.; Kandil H.; Palani A.; Salvio G.; Mostafa T.; Rajmil O.; Banihani S.A.; Schon S.; Le T.V.; Birowo P.; Çeker G.; Alvarez J.; Molina J.M.C.; Ho C.C.K.; Calogero A.E.; Khalafalla K.; Duran M.B.; Kuroda S.; Colpi G.M.; Zini A.; Anagnostopoulou C.; Pescatori E.; Chung E.; Caroppo E.; Dimitriadis F.; Pinggera G.-M.; Busetto G.M.; Balercia G.; Elbardisi H.; Taniguchi H.; Park H.J.; Rosas I.M.; de la Rosette J.; Ramsay J.; Bowa K.; Simopoulou M.; Rodriguez M.G.; Sabbaghian M.; Martinez M.; Gilani M.A.S.; Al-Marhoon M.S.; Kosgi R.; Cannarella R.; Micic S.; Fukuhara S.; Parekattil S.; Jindal S.; Abdel-Meguid T.A.-A.; Morimoto Y.; Shah R.
Sperm vitality and necrozoospermia: diagnosis, management, and results of a global survey of clinical practice
author_facet Agarwal A.; Sharma R.K.; Gupta S.; Boitrelle F.; Finelli R.; Parekh N.; Durairajanayagam D.; Saleh R.; Arafa M.; Cho C.L.; Farkouh A.; Rambhatla A.; Henkel R.; Vogiatzi P.; Tadros N.; Kavoussi P.; Ko E.; Leisegang K.; Kandil H.; Palani A.; Salvio G.; Mostafa T.; Rajmil O.; Banihani S.A.; Schon S.; Le T.V.; Birowo P.; Çeker G.; Alvarez J.; Molina J.M.C.; Ho C.C.K.; Calogero A.E.; Khalafalla K.; Duran M.B.; Kuroda S.; Colpi G.M.; Zini A.; Anagnostopoulou C.; Pescatori E.; Chung E.; Caroppo E.; Dimitriadis F.; Pinggera G.-M.; Busetto G.M.; Balercia G.; Elbardisi H.; Taniguchi H.; Park H.J.; Rosas I.M.; de la Rosette J.; Ramsay J.; Bowa K.; Simopoulou M.; Rodriguez M.G.; Sabbaghian M.; Martinez M.; Gilani M.A.S.; Al-Marhoon M.S.; Kosgi R.; Cannarella R.; Micic S.; Fukuhara S.; Parekattil S.; Jindal S.; Abdel-Meguid T.A.-A.; Morimoto Y.; Shah R.
author_sort Agarwal A.; Sharma R.K.; Gupta S.; Boitrelle F.; Finelli R.; Parekh N.; Durairajanayagam D.; Saleh R.; Arafa M.; Cho C.L.; Farkouh A.; Rambhatla A.; Henkel R.; Vogiatzi P.; Tadros N.; Kavoussi P.; Ko E.; Leisegang K.; Kandil H.; Palani A.; Salvio G.; Mostafa T.; Rajmil O.; Banihani S.A.; Schon S.; Le T.V.; Birowo P.; Çeker G.; Alvarez J.; Molina J.M.C.; Ho C.C.K.; Calogero A.E.; Khalafalla K.; Duran M.B.; Kuroda S.; Colpi G.M.; Zini A.; Anagnostopoulou C.; Pescatori E.; Chung E.; Caroppo E.; Dimitriadis F.; Pinggera G.-M.; Busetto G.M.; Balercia G.; Elbardisi H.; Taniguchi H.; Park H.J.; Rosas I.M.; de la Rosette J.; Ramsay J.; Bowa K.; Simopoulou M.; Rodriguez M.G.; Sabbaghian M.; Martinez M.; Gilani M.A.S.; Al-Marhoon M.S.; Kosgi R.; Cannarella R.; Micic S.; Fukuhara S.; Parekattil S.; Jindal S.; Abdel-Meguid T.A.-A.; Morimoto Y.; Shah R.
title Sperm vitality and necrozoospermia: diagnosis, management, and results of a global survey of clinical practice
title_short Sperm vitality and necrozoospermia: diagnosis, management, and results of a global survey of clinical practice
title_full Sperm vitality and necrozoospermia: diagnosis, management, and results of a global survey of clinical practice
title_fullStr Sperm vitality and necrozoospermia: diagnosis, management, and results of a global survey of clinical practice
title_full_unstemmed Sperm vitality and necrozoospermia: diagnosis, management, and results of a global survey of clinical practice
title_sort Sperm vitality and necrozoospermia: diagnosis, management, and results of a global survey of clinical practice
publishDate 2022
container_title World Journal of Men's Health
container_volume 40
container_issue 2
doi_str_mv 10.5534/wjmh.210149
url https://www.scopus.com/inward/record.uri?eid=2-s2.0-85118736952&doi=10.5534%2fwjmh.210149&partnerID=40&md5=776ce144f9bcad4451df84b64415ccd7
description Sperm vitality testing is a basic semen examination that has been described in the World Health Organization (WHO) Laboratory Manual for the Examination and Processing of Human Semen from its primary edition, 40 years ago. Several methods can be used to test sperm vitality, such as the eosin-nigrosin (E-N) stain or the hypoosmotic swelling (HOS) test. In the 6th (2021) edition of the WHO Laboratory Manual, sperm vitality assessment is mainly recommended if the total motility is less than 40%. Hence, a motile spermatozoon is considered alive, however, in certain conditions an immotile spermatozoon can also be alive. Therefore, the differentiation between asthenozoospermia (pathological decrease in sperm motility) and necrozoospermia (pathological decrease in sperm vitality) is important in directing further investigation and management of infertile patients. The causes leading to necrozoospermia are diverse and can either be local or general, testicular or extra-testicular. The andrological management of necrozoospermia depends on its etiology. However, there is no standardized treatment available presently and practice varies among clinicians. In this study, we report the results of a global survey to understand current practices regarding the physician order of sperm vitality tests as well as the management practices for necrozoospermia. Laboratory and clinical scenarios are presented to guide the reader in the management of necrozoospermia with the overall objective of establishing a benchmark ranging from the diagnosis of necrozoospermia by sperm vitality testing to its clinical management. Copyright © 2021 Korean Society for Sexual Medicine and Andrology
publisher Korean Society for Sexual Medicine and Andrology
issn 22874208
language English
format Review
accesstype All Open Access; Gold Open Access
record_format scopus
collection Scopus
_version_ 1812871798732095488

Similar Items

  • Antisperm Antibody Testing: A Comprehensive Review of Its Role in the Management of Immunological Male Infertility and Results of a Global Survey of Clinical Practices
    by: Gupta S.; Sharma R.; Agarwal A.; Boitrelle F.; Finelli R.; Farkouh A.; Saleh R.; Abdel-Meguid T.A.-A.; Gül M.; Zilaitiene B.; Ko E.; Rambhatla A.; Zini A.; Leisegang K.; Kuroda S.; Henkel R.; Cannarella R.; Palani A.; Cho C.-L.; Ho C.C.K.; Zylbersztejn D.S.; Pescatori E.; Chung E.; Dimitriadis F.; Pinggera G.-M.; Busetto G.M.; Balercia G.; Salvio G.; Colpi G.M.; Çeker G.; Taniguchi H.; Kandil H.; Park H.J.; Rosas I.M.; de la Rosette J.; Cardoso J.P.G.; Ramsay J.; Alvarez J.; Molina J.M.C.; Khalafalla K.; Bowa K.; Tremellen K.; Evgeni E.; Rocco L.; Peña M.G.R.; Sabbaghian M.; Martinez M.; Arafa M.; Al-Marhoon M.S.; Tadros N.; Garrido N.; Rajmil O.; Sengupta P.; Vogiatzi P.; Kavoussi P.; Birowo P.; Kosgi R.; Bani-Hani S.; Micic S.; Parekattil S.; Jindal S.; Le T.V.; Mostafa T.; Toprak T.; Morimoto Y.; Malhotra V.; Aghamajidi A.; Durairajanayagam D.; Shah R.
    Published: (2022)
  • Relevance of leukocytospermia and semen culture and its true place in diagnosing and treating male infertility
    by: Sharma R.; Gupta S.; Agarwal A.; Henkel R.; Finelli R.; Parekh N.; Saleh R.; Arafa M.; Ko E.; Zini A.; Tadros N.; Shah R.; Ambar R.F.; Elbardisi H.; Sengupta P.; Martinez M.; Boitrelle F.; Simopoulou M.; Vogiatzi P.; Gosalvez J.; Kavoussi P.; Kandil H.; Palani A.; Peña M.R.; Rajmil O.; Busetto G.M.; Anagnostopoulou C.; Micic S.; Alves M.G.; Rocco L.; Mostafa T.; Alvarez J.G.; Jindal S.; Sallam H.N.; Rosas I.M.; Lewis S.E.M.; AlSaid S.; Altan M.; Park H.J.; Ramsay J.; Parekattil S.; Sabbaghian M.; Tremellen K.; Khalafalla K.; Durairajanayagam D.; Colpi G.M.
    Published: (2021)
  • Post-Vasectomy Semen Analysis: Optimizing Laboratory Procedures and Test Interpretation through a Clinical Audit and Global Survey of Practices
    by: Agarwal A.; Gupta S.; Sharma R.K.; Finelli R.; Kuroda S.; Vij S.C.; Boitrelle F.; Kavoussi P.; Rambhatla A.; Saleh R.; Chung E.; Mostafa T.; Zini A.; Ko E.; Parekh N.; Martinez M.; Arafa M.; Tadros N.; de la Rosette J.; Le T.V.; Rajmil O.; Kandil H.; Blecher G.; Liguori G.; Caroppo E.; Ho C.C.K.; Altman A.; Bajic P.; Goldfarb D.; Gill B.; Zylbersztejn D.S.; Corral Molina J.M.; Gava M.M.; Greco Cardoso J.P.; Kosgi R.; Çeker G.; Zilaitiene B.; Pescatori E.; Borges E.; Kusuma Duarsa G.W.; Pinggera G.-M.; Busetto G.M.; Balercia G.; Franco G.; Çalik G.; Sallam H.N.; Park H.J.; Ramsay J.; Alvarez J.; Khalafalla K.; Bowa K.; Hakim L.; Simopoulou M.; Rodriguez M.G.; Sabbaghian M.; Elbardisi H.; Timpano M.; Altan M.; Elkhouly M.; Al-Marhoon M.S.; Sadighi Gilani M.A.; Soebadi M.A.; Nasr-Esfahani M.H.; Garrido N.; Vogiatzi P.; Birowo P.; Patel P.; Javed Q.; Ambar R.F.; Adriansjah R.; AlSaid S.; Micic S.; Lewis S.E.; Mutambirwa S.; Fukuhara S.; Parekattil S.; Ahn S.T.; Jindal S.; Takeshima T.; Puigvert A.; Amano T.; Barrett T.; Toprak T.; Malhotra V.; Atmoko W.; Yumura Y.; Morimoto Y.; Negris Lima T.F.; Kunz Y.; Kato Y.; Umemoto Y.; Colpi G.M.; Durairajanayagam D.; Shah R.
    Published: (2022)
  • Molecular basis of specificity and deamidation of eIF4A by Burkholderia Lethal Factor 1
    by: Mobbs G.W.; Aziz A.A.; Dix S.R.; Blackburn G.M.; Sedelnikova S.E.; Minshull T.C.; Dickman M.J.; Baker P.J.; Nathan S.; Raih M.F.; Rice D.W.
    Published: (2022)
  • Sperm morphology assessment in the era of intracytoplasmic sperm injection: Reliable results require focus on standardization, quality control, and training
    by: Agarwal A.; Sharma R.; Gupta S.; Finelli R.; Parekh N.; Selvam M.K.P.; Henkel R.; Durairajanayagam D.; Pompeu C.; Madani S.; Belo A.; Singh N.; Covarrubias S.; Darbandi S.; Sadeghi R.; Darbandi M.; Vogiatzi P.; Boitrelle F.; Simopoulou M.; Saleh R.; Arafa M.; Majzoub A.; Kandil H.; Zini A.; Ko E.; Alvarez J.G.; Martinez M.; Ramsay J.; Jindal S.; Busetto G.M.; Sallam H.; Maldonado I.; Anagnostopoulou C.; Alves M.G.; Sengupta P.; Gilany K.; Evenson D.P.; Lewis S.E.M.; Gosalvez J.; Ambar R.F.; Shah R.
    Published: (2021)