Screening of ligninolytic fungi for bioremediation of dyes

Water pollution is a growing concern worldwide. One of the main causes of water pollution includes the textile industry which produces a large amount of wastewater every day. This wastewater is known to contain dyes that are recalcitrant and hard to treat. In order to solve this problem, bioremediat...

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Published in:Asia-Pacific Journal of Molecular Biology and Biotechnology
Main Author: Muliyadi S.A.; Mulok E.T.Z.; Hussein N.H.; Nor R.M.
Format: Article
Language:English
Published: University of Malaya 2021
Online Access:https://www.scopus.com/inward/record.uri?eid=2-s2.0-85107860277&doi=10.35118%2fapjmbb.2021.029.2.04&partnerID=40&md5=af28d507a9f574d20e37f63a1f228da3
id 2-s2.0-85107860277
spelling 2-s2.0-85107860277
Muliyadi S.A.; Mulok E.T.Z.; Hussein N.H.; Nor R.M.
Screening of ligninolytic fungi for bioremediation of dyes
2021
Asia-Pacific Journal of Molecular Biology and Biotechnology
29
2
10.35118/apjmbb.2021.029.2.04
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85107860277&doi=10.35118%2fapjmbb.2021.029.2.04&partnerID=40&md5=af28d507a9f574d20e37f63a1f228da3
Water pollution is a growing concern worldwide. One of the main causes of water pollution includes the textile industry which produces a large amount of wastewater every day. This wastewater is known to contain dyes that are recalcitrant and hard to treat. In order to solve this problem, bioremediation using ligninolytic fungi is commonly used for the ligninolytic enzymes which are able to break down the dyes. In this study, samples were collected from decaying woods and soils in the vicinity of UiTM Puncak Alam forests. A total of 20 fungal isolates were tested for ligninolytic enzyme production. Out of the 20 isolates, 13 were found to produce lignin peroxidase and manganese peroxidase, but only one produced laccase. The isolate that produced all three enzymes was used for DNA isolation and identified using amplification of the ITS region by PCR. The isolate was identified as Trichoderma asperellum, a soft rot fungal species which is renowned for its role in bioremediation as a biosorbent. © 2021, University of Malaya. All rights reserved.
University of Malaya
1287451
English
Article
All Open Access; Gold Open Access
author Muliyadi S.A.; Mulok E.T.Z.; Hussein N.H.; Nor R.M.
spellingShingle Muliyadi S.A.; Mulok E.T.Z.; Hussein N.H.; Nor R.M.
Screening of ligninolytic fungi for bioremediation of dyes
author_facet Muliyadi S.A.; Mulok E.T.Z.; Hussein N.H.; Nor R.M.
author_sort Muliyadi S.A.; Mulok E.T.Z.; Hussein N.H.; Nor R.M.
title Screening of ligninolytic fungi for bioremediation of dyes
title_short Screening of ligninolytic fungi for bioremediation of dyes
title_full Screening of ligninolytic fungi for bioremediation of dyes
title_fullStr Screening of ligninolytic fungi for bioremediation of dyes
title_full_unstemmed Screening of ligninolytic fungi for bioremediation of dyes
title_sort Screening of ligninolytic fungi for bioremediation of dyes
publishDate 2021
container_title Asia-Pacific Journal of Molecular Biology and Biotechnology
container_volume 29
container_issue 2
doi_str_mv 10.35118/apjmbb.2021.029.2.04
url https://www.scopus.com/inward/record.uri?eid=2-s2.0-85107860277&doi=10.35118%2fapjmbb.2021.029.2.04&partnerID=40&md5=af28d507a9f574d20e37f63a1f228da3
description Water pollution is a growing concern worldwide. One of the main causes of water pollution includes the textile industry which produces a large amount of wastewater every day. This wastewater is known to contain dyes that are recalcitrant and hard to treat. In order to solve this problem, bioremediation using ligninolytic fungi is commonly used for the ligninolytic enzymes which are able to break down the dyes. In this study, samples were collected from decaying woods and soils in the vicinity of UiTM Puncak Alam forests. A total of 20 fungal isolates were tested for ligninolytic enzyme production. Out of the 20 isolates, 13 were found to produce lignin peroxidase and manganese peroxidase, but only one produced laccase. The isolate that produced all three enzymes was used for DNA isolation and identified using amplification of the ITS region by PCR. The isolate was identified as Trichoderma asperellum, a soft rot fungal species which is renowned for its role in bioremediation as a biosorbent. © 2021, University of Malaya. All rights reserved.
publisher University of Malaya
issn 1287451
language English
format Article
accesstype All Open Access; Gold Open Access
record_format scopus
collection Scopus
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