Ficus deltoidea suppresses endothelial activation, inflammation, monocytes adhesion and oxidative stress via NF-κB and eNOS pathways in stimulated human coronary artery endothelial cells

BACKGROUND: Ficus deltoidea (FD) has been shown to have antidiabetic, anti-inflammatory, antinociceptive and antioxidant properties. However, its effects on key events in the pathogenesis of atherosclerosis are unknown. AIM: To investigate the endothelial activation, inflammation, monocyte-endotheli...

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Published in:BMC complementary medicine and therapies
Main Author: Mohd Ariff A.; Abu Bakar N.A.; Abd Muid S.; Omar E.; Ismail N.H.; Ali A.M.; Mohd Kasim N.A.; Mohd Nawawi H.
Format: Article
Language:English
Published: NLM (Medline) 2020
Online Access:https://www.scopus.com/inward/record.uri?eid=2-s2.0-85089582076&doi=10.1186%2fs12906-020-2844-6&partnerID=40&md5=66a2406b251345aa7377f361a6c92aee
id 2-s2.0-85089582076
spelling 2-s2.0-85089582076
Mohd Ariff A.; Abu Bakar N.A.; Abd Muid S.; Omar E.; Ismail N.H.; Ali A.M.; Mohd Kasim N.A.; Mohd Nawawi H.
Ficus deltoidea suppresses endothelial activation, inflammation, monocytes adhesion and oxidative stress via NF-κB and eNOS pathways in stimulated human coronary artery endothelial cells
2020
BMC complementary medicine and therapies
20
1
10.1186/s12906-020-2844-6
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85089582076&doi=10.1186%2fs12906-020-2844-6&partnerID=40&md5=66a2406b251345aa7377f361a6c92aee
BACKGROUND: Ficus deltoidea (FD) has been shown to have antidiabetic, anti-inflammatory, antinociceptive and antioxidant properties. However, its effects on key events in the pathogenesis of atherosclerosis are unknown. AIM: To investigate the endothelial activation, inflammation, monocyte-endothelial cell binding and oxidative stress effects of four FD varieties. METHODS: Human coronary artery endothelial cells (HCAEC) were incubated with different concentrations of aqueous ethanolic extracts of FD var. trengganuensis (FDT), var. kunstleri (FDK), var. deltoidea (FDD) and var. intermedia (FDI), together with LPS. Protein and gene expression of vascular cell adhesion molecule-1 (VCAM-1), intercellular cell adhesion molecule-1 (ICAM-1), endothelial-leukocyte adhesion molecule-1 (E-selectin), interleukin-6 (IL-6), Nuclear factor-κB (NF-κB) p50 and p65 and endothelial nitric oxide synthase (eNOS) were measured using ELISA and QuantiGene plex, respectively. Adhesion of monocyte to HCAEC and formation of reactive oxygen species (ROS) were detected by Rose Bengal staining and 2'-7'-dichlorofluorescein diacetate (DCFH-DA) assay. RESULTS: FDK exhibited the highest inhibition of biomarkers in relation to endothelial activation and inflammation, second in reducing monocyte binding (17.3%) compared to other varieties. FDK (25.6%) was also the most potent at decreasing ROS production. CONCLUSION: FD has anti-atherogenic effects, possibly mediated by NF-κB and eNOS pathways; with FDK being the most potent variety. It is potentially beneficial in mitigating atherogenesis.
NLM (Medline)
26627671
English
Article
All Open Access; Gold Open Access
author Mohd Ariff A.; Abu Bakar N.A.; Abd Muid S.; Omar E.; Ismail N.H.; Ali A.M.; Mohd Kasim N.A.; Mohd Nawawi H.
spellingShingle Mohd Ariff A.; Abu Bakar N.A.; Abd Muid S.; Omar E.; Ismail N.H.; Ali A.M.; Mohd Kasim N.A.; Mohd Nawawi H.
Ficus deltoidea suppresses endothelial activation, inflammation, monocytes adhesion and oxidative stress via NF-κB and eNOS pathways in stimulated human coronary artery endothelial cells
author_facet Mohd Ariff A.; Abu Bakar N.A.; Abd Muid S.; Omar E.; Ismail N.H.; Ali A.M.; Mohd Kasim N.A.; Mohd Nawawi H.
author_sort Mohd Ariff A.; Abu Bakar N.A.; Abd Muid S.; Omar E.; Ismail N.H.; Ali A.M.; Mohd Kasim N.A.; Mohd Nawawi H.
title Ficus deltoidea suppresses endothelial activation, inflammation, monocytes adhesion and oxidative stress via NF-κB and eNOS pathways in stimulated human coronary artery endothelial cells
title_short Ficus deltoidea suppresses endothelial activation, inflammation, monocytes adhesion and oxidative stress via NF-κB and eNOS pathways in stimulated human coronary artery endothelial cells
title_full Ficus deltoidea suppresses endothelial activation, inflammation, monocytes adhesion and oxidative stress via NF-κB and eNOS pathways in stimulated human coronary artery endothelial cells
title_fullStr Ficus deltoidea suppresses endothelial activation, inflammation, monocytes adhesion and oxidative stress via NF-κB and eNOS pathways in stimulated human coronary artery endothelial cells
title_full_unstemmed Ficus deltoidea suppresses endothelial activation, inflammation, monocytes adhesion and oxidative stress via NF-κB and eNOS pathways in stimulated human coronary artery endothelial cells
title_sort Ficus deltoidea suppresses endothelial activation, inflammation, monocytes adhesion and oxidative stress via NF-κB and eNOS pathways in stimulated human coronary artery endothelial cells
publishDate 2020
container_title BMC complementary medicine and therapies
container_volume 20
container_issue 1
doi_str_mv 10.1186/s12906-020-2844-6
url https://www.scopus.com/inward/record.uri?eid=2-s2.0-85089582076&doi=10.1186%2fs12906-020-2844-6&partnerID=40&md5=66a2406b251345aa7377f361a6c92aee
description BACKGROUND: Ficus deltoidea (FD) has been shown to have antidiabetic, anti-inflammatory, antinociceptive and antioxidant properties. However, its effects on key events in the pathogenesis of atherosclerosis are unknown. AIM: To investigate the endothelial activation, inflammation, monocyte-endothelial cell binding and oxidative stress effects of four FD varieties. METHODS: Human coronary artery endothelial cells (HCAEC) were incubated with different concentrations of aqueous ethanolic extracts of FD var. trengganuensis (FDT), var. kunstleri (FDK), var. deltoidea (FDD) and var. intermedia (FDI), together with LPS. Protein and gene expression of vascular cell adhesion molecule-1 (VCAM-1), intercellular cell adhesion molecule-1 (ICAM-1), endothelial-leukocyte adhesion molecule-1 (E-selectin), interleukin-6 (IL-6), Nuclear factor-κB (NF-κB) p50 and p65 and endothelial nitric oxide synthase (eNOS) were measured using ELISA and QuantiGene plex, respectively. Adhesion of monocyte to HCAEC and formation of reactive oxygen species (ROS) were detected by Rose Bengal staining and 2'-7'-dichlorofluorescein diacetate (DCFH-DA) assay. RESULTS: FDK exhibited the highest inhibition of biomarkers in relation to endothelial activation and inflammation, second in reducing monocyte binding (17.3%) compared to other varieties. FDK (25.6%) was also the most potent at decreasing ROS production. CONCLUSION: FD has anti-atherogenic effects, possibly mediated by NF-κB and eNOS pathways; with FDK being the most potent variety. It is potentially beneficial in mitigating atherogenesis.
publisher NLM (Medline)
issn 26627671
language English
format Article
accesstype All Open Access; Gold Open Access
record_format scopus
collection Scopus
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