BSA Binding, molecular docking and in vitro biological screening of some new 1, 2, 4-triazole heterocycles bearing azinane nucleus

• Published in: Pakistan Journal of Pharmaceutical Sciences
• Main Author: Iqbal J.; Aziz-Ur-Rehman; Abbasi M.A.; Siddiqui S.Z.; Khalid H.; Laulloo S.J.; Chohan T.A.; Rasool S.; Ali Shah S.A.
• Format: Article
• Language: English
• Published: Pakistan Journal of Pharmaceutical Sciences 2020
• Online Access: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85077920867&doi=10.36721%2fPJPS.2020.33.1.REG.149-160.1&partnerID=40&md5=5a5091bd9e7e9d2ef6cf1ce342b95d87

A series of new compounds (5a-q), derived from 5-(1-(4-nitrophenylsulfonyl) piperidin-4-yl)-4-phenyl-4H-1,2,4-triazole-3-thiol (3) were proficiently synthesized to evaluate their biological activities. 1-(4-Nitrophenylsulfonyl) piperidine-4-carbohydrazide (2) was refluxed with phenylisothiocyanate to yield an adduct which was cyclized to compound 3 by reflux reaction with 10 % potassium hydroxide. The targeted compounds 5a-q, were synthesized by stirring alkyl/aralkyl halides (4a-q) and compound 3 in a polar aprotic solvent. 1H-NMR, 13C-NMR, EI-MS and IR spectral techniques were employed to confirm the structures of all the synthesized compounds. The compounds were biologically evaluated for BSA binding studies followed by anti-bacterial, anti-inflammatory and acetylcholinesterase (AChE) activities. The active sites responsible for the best AChE inhibition were identified through molecular docking studies. Compound 5e bearing 4-chlorobenzyl moiety found most active antibacterial and anti-inflammatory agent among the synthesized compounds. The whole library of synthesized compounds except compounds 5d and 5f was found highly active for AChE inhibition and recommended for in vivo studies so that their therapeutic applications may come in utilization. © 2020 Pakistan Journal of Pharmaceutical Sciences. All rights reserved.