| Summary: | Palm kernel cake (PKC) proteins were hydrolysed in the presence of seven proteolytic enzymes. The antioxidant and antihypertensive activities of each protein hydrolysate were evaluated. Papain-generated protein hydrolysate showed the highest antioxidant effects measured by radical scavenging activity (65%), iron (Fe)(II)-chelating activity (65%), and antihypertensive activity (71%). The results revealed a strong correlation between the antioxidant activity of the protein hydrolysates with angiotensin-converting enzyme (ACE) inhibitory activity (R2 = 0.69-0.98), except for trypsin-generated hydrolysate (R2 = 0.22). The most potent protein hydrolysate was fractionated and profiled using reversed-phase high-performance liquid chromatography and isoelectric focusing; peptides were subsequently isolated and identified by tandem mass spectrometry. The individual peptides were evaluated for antihypertension potential. A positive correlation was identified between radical scavenging activity and Fe(II)-chelating activity together and ACE inhibitory activity with R2 = 0.69-0.98. The findings indicate that there was a positive relationship between the antioxidant and antihypertensive activities of protein hydrolysates and bioactive peptides from PKC proteins. © 2019, Universiti Putra Malaysia.
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