Method development and validation for quantification of cortisol and cortisone using ultra-high performance liquid chromatography-tandem mass spectrometry; [Pembangunan dan pengesahan kaedah untuk kuantifikasi kortisol dan kortison menggunakan kromatografi cecair berprestasi ultra tinggi-spektrometri jisim gabungan]

Cortisol and its metabolite cortisone have been used as a biological marker of stress in human psychobiological studies. Cortisol and cortisone quantitation may help to examine physiological responses towards stress. A highly sensitive and selective ultra-high performance liquid chromatography tande...

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Published in:Malaysian Journal of Analytical Sciences
Main Author: Hazirah A.A.; Rofiee M.S.; Jam M.Z.J.; Jamari M.H.; Janor R.M.; James R.J.; Kek T.L.; Salleh M.Z.
Format: Article
Language:English
Published: Malaysian Society of Analytical Sciences 2019
Online Access:https://www.scopus.com/inward/record.uri?eid=2-s2.0-85074728235&doi=10.17576%2fmjas-2019-2302-17&partnerID=40&md5=723f6a4b06489e07d9b9966882a770f7
id 2-s2.0-85074728235
spelling 2-s2.0-85074728235
Hazirah A.A.; Rofiee M.S.; Jam M.Z.J.; Jamari M.H.; Janor R.M.; James R.J.; Kek T.L.; Salleh M.Z.
Method development and validation for quantification of cortisol and cortisone using ultra-high performance liquid chromatography-tandem mass spectrometry; [Pembangunan dan pengesahan kaedah untuk kuantifikasi kortisol dan kortison menggunakan kromatografi cecair berprestasi ultra tinggi-spektrometri jisim gabungan]
2019
Malaysian Journal of Analytical Sciences
23
2
10.17576/mjas-2019-2302-17
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85074728235&doi=10.17576%2fmjas-2019-2302-17&partnerID=40&md5=723f6a4b06489e07d9b9966882a770f7
Cortisol and its metabolite cortisone have been used as a biological marker of stress in human psychobiological studies. Cortisol and cortisone quantitation may help to examine physiological responses towards stress. A highly sensitive and selective ultra-high performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) method was developed for simultaneous quantitation of cortisol and cortisone in human serum. These two compounds were chromatographically separated using Agilent ZORBAX Eclipse Plus C18 Rapid Resolution HD column (2.1 mm × 50 mm, 1.8 µm) and were eluted with (A) 0.1% formic acid in water and (B) methanol mobile phase. The sample injection volume was 2 µL and the flow rate was set at 0.3 mL/min. The analytes were determined in positive ionization mode and quantitated by multiple-reaction monitoring (MRM) mode. The method was linear from 7.8 to 500 ng/mL for cortisol (r2 = 0.986) and cortisone (r2 = 0.990). The precision, accuracy, and recovery of the method for cortisol ranged from 1.98-10.46%, 90.68-91.94%, 90.38-93.88%, and cortisone ranged from 1.01-11.36%, 93.12-94.79% and 90.48-95.90%, respectively. The developed method has met the quality standards of European Medicines Agency (EMA). © 2019, Malaysian Society of Analytical Sciences. All rights reserved.
Malaysian Society of Analytical Sciences
13942506
English
Article
All Open Access; Gold Open Access
author Hazirah A.A.; Rofiee M.S.; Jam M.Z.J.; Jamari M.H.; Janor R.M.; James R.J.; Kek T.L.; Salleh M.Z.
spellingShingle Hazirah A.A.; Rofiee M.S.; Jam M.Z.J.; Jamari M.H.; Janor R.M.; James R.J.; Kek T.L.; Salleh M.Z.
Method development and validation for quantification of cortisol and cortisone using ultra-high performance liquid chromatography-tandem mass spectrometry; [Pembangunan dan pengesahan kaedah untuk kuantifikasi kortisol dan kortison menggunakan kromatografi cecair berprestasi ultra tinggi-spektrometri jisim gabungan]
author_facet Hazirah A.A.; Rofiee M.S.; Jam M.Z.J.; Jamari M.H.; Janor R.M.; James R.J.; Kek T.L.; Salleh M.Z.
author_sort Hazirah A.A.; Rofiee M.S.; Jam M.Z.J.; Jamari M.H.; Janor R.M.; James R.J.; Kek T.L.; Salleh M.Z.
title Method development and validation for quantification of cortisol and cortisone using ultra-high performance liquid chromatography-tandem mass spectrometry; [Pembangunan dan pengesahan kaedah untuk kuantifikasi kortisol dan kortison menggunakan kromatografi cecair berprestasi ultra tinggi-spektrometri jisim gabungan]
title_short Method development and validation for quantification of cortisol and cortisone using ultra-high performance liquid chromatography-tandem mass spectrometry; [Pembangunan dan pengesahan kaedah untuk kuantifikasi kortisol dan kortison menggunakan kromatografi cecair berprestasi ultra tinggi-spektrometri jisim gabungan]
title_full Method development and validation for quantification of cortisol and cortisone using ultra-high performance liquid chromatography-tandem mass spectrometry; [Pembangunan dan pengesahan kaedah untuk kuantifikasi kortisol dan kortison menggunakan kromatografi cecair berprestasi ultra tinggi-spektrometri jisim gabungan]
title_fullStr Method development and validation for quantification of cortisol and cortisone using ultra-high performance liquid chromatography-tandem mass spectrometry; [Pembangunan dan pengesahan kaedah untuk kuantifikasi kortisol dan kortison menggunakan kromatografi cecair berprestasi ultra tinggi-spektrometri jisim gabungan]
title_full_unstemmed Method development and validation for quantification of cortisol and cortisone using ultra-high performance liquid chromatography-tandem mass spectrometry; [Pembangunan dan pengesahan kaedah untuk kuantifikasi kortisol dan kortison menggunakan kromatografi cecair berprestasi ultra tinggi-spektrometri jisim gabungan]
title_sort Method development and validation for quantification of cortisol and cortisone using ultra-high performance liquid chromatography-tandem mass spectrometry; [Pembangunan dan pengesahan kaedah untuk kuantifikasi kortisol dan kortison menggunakan kromatografi cecair berprestasi ultra tinggi-spektrometri jisim gabungan]
publishDate 2019
container_title Malaysian Journal of Analytical Sciences
container_volume 23
container_issue 2
doi_str_mv 10.17576/mjas-2019-2302-17
url https://www.scopus.com/inward/record.uri?eid=2-s2.0-85074728235&doi=10.17576%2fmjas-2019-2302-17&partnerID=40&md5=723f6a4b06489e07d9b9966882a770f7
description Cortisol and its metabolite cortisone have been used as a biological marker of stress in human psychobiological studies. Cortisol and cortisone quantitation may help to examine physiological responses towards stress. A highly sensitive and selective ultra-high performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) method was developed for simultaneous quantitation of cortisol and cortisone in human serum. These two compounds were chromatographically separated using Agilent ZORBAX Eclipse Plus C18 Rapid Resolution HD column (2.1 mm × 50 mm, 1.8 µm) and were eluted with (A) 0.1% formic acid in water and (B) methanol mobile phase. The sample injection volume was 2 µL and the flow rate was set at 0.3 mL/min. The analytes were determined in positive ionization mode and quantitated by multiple-reaction monitoring (MRM) mode. The method was linear from 7.8 to 500 ng/mL for cortisol (r2 = 0.986) and cortisone (r2 = 0.990). The precision, accuracy, and recovery of the method for cortisol ranged from 1.98-10.46%, 90.68-91.94%, 90.38-93.88%, and cortisone ranged from 1.01-11.36%, 93.12-94.79% and 90.48-95.90%, respectively. The developed method has met the quality standards of European Medicines Agency (EMA). © 2019, Malaysian Society of Analytical Sciences. All rights reserved.
publisher Malaysian Society of Analytical Sciences
issn 13942506
language English
format Article
accesstype All Open Access; Gold Open Access
record_format scopus
collection Scopus
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