Quadrupole-Time-of-Flight Mass Spectrometric Identification of Hemoglobin Subunits α, β, γ and δ in Unknown Peaks of High Performance Liquid Chromatography of Hemoglobin in β-Thalassemias
This is the first report of quadrupole time-of-flight (Q-TOF) mass spectrometric identification of the hemoglobin (Hb) subunits, α, β, δ and γ peptides, derived from enzymatic-digestion of proteins in the early unknown peaks of the cation exchange chromatography of Hb. The objectives were to identif...
| Published in: | Hemoglobin |
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| Format: | Article |
| Language: | English |
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Taylor and Francis Ltd
2019
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| Online Access: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85068914289&doi=10.1080%2f03630269.2019.1632893&partnerID=40&md5=d0a666ecbe676cf7ea5f3b27e81a67ef |
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2-s2.0-85068914289 |
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2-s2.0-85068914289 Abdullah U.Y.H.; Ibrahim H.M.; Mahmud N.B.; Salleh M.Z.; Kek T.L.; Noorizhab M.N.F.B.; Jassim H.M.; Othman I.; Zainal Abidin S.A.; Zilfalil B.A.; Wilairat P.; Fucharoen S. Quadrupole-Time-of-Flight Mass Spectrometric Identification of Hemoglobin Subunits α, β, γ and δ in Unknown Peaks of High Performance Liquid Chromatography of Hemoglobin in β-Thalassemias 2019 Hemoglobin 43 3 10.1080/03630269.2019.1632893 https://www.scopus.com/inward/record.uri?eid=2-s2.0-85068914289&doi=10.1080%2f03630269.2019.1632893&partnerID=40&md5=d0a666ecbe676cf7ea5f3b27e81a67ef This is the first report of quadrupole time-of-flight (Q-TOF) mass spectrometric identification of the hemoglobin (Hb) subunits, α, β, δ and γ peptides, derived from enzymatic-digestion of proteins in the early unknown peaks of the cation exchange chromatography of Hb. The objectives were to identify the unknown high performance liquid chromatography (HPLC) peaks in healthy subjects and in patients with β-thalassemia (β-thal). The results demonstrate the existence of pools of free globin chains in red blood cells (RBCs). The α-, β-, δ- and γ-globin peptides were identified in the unknown HPLC peaks. The quantification and role of the free globin pool in patients with β-thal requires further investigation. Identification of all types of Hb subunits in the retention time (RT) before 1 min. suggests that altered Hbs is the nature of these fast-eluting peaks. Relevancy of thalassemias to the protein-aggregation disorders will require review of the role of free globin in the pathology of the disease. © 2019, © 2019 Informa UK Limited, trading as Taylor & Francis Group. Taylor and Francis Ltd 3630269 English Article |
| author |
Abdullah U.Y.H.; Ibrahim H.M.; Mahmud N.B.; Salleh M.Z.; Kek T.L.; Noorizhab M.N.F.B.; Jassim H.M.; Othman I.; Zainal Abidin S.A.; Zilfalil B.A.; Wilairat P.; Fucharoen S. |
| spellingShingle |
Abdullah U.Y.H.; Ibrahim H.M.; Mahmud N.B.; Salleh M.Z.; Kek T.L.; Noorizhab M.N.F.B.; Jassim H.M.; Othman I.; Zainal Abidin S.A.; Zilfalil B.A.; Wilairat P.; Fucharoen S. Quadrupole-Time-of-Flight Mass Spectrometric Identification of Hemoglobin Subunits α, β, γ and δ in Unknown Peaks of High Performance Liquid Chromatography of Hemoglobin in β-Thalassemias |
| author_facet |
Abdullah U.Y.H.; Ibrahim H.M.; Mahmud N.B.; Salleh M.Z.; Kek T.L.; Noorizhab M.N.F.B.; Jassim H.M.; Othman I.; Zainal Abidin S.A.; Zilfalil B.A.; Wilairat P.; Fucharoen S. |
| author_sort |
Abdullah U.Y.H.; Ibrahim H.M.; Mahmud N.B.; Salleh M.Z.; Kek T.L.; Noorizhab M.N.F.B.; Jassim H.M.; Othman I.; Zainal Abidin S.A.; Zilfalil B.A.; Wilairat P.; Fucharoen S. |
| title |
Quadrupole-Time-of-Flight Mass Spectrometric Identification of Hemoglobin Subunits α, β, γ and δ in Unknown Peaks of High Performance Liquid Chromatography of Hemoglobin in β-Thalassemias |
| title_short |
Quadrupole-Time-of-Flight Mass Spectrometric Identification of Hemoglobin Subunits α, β, γ and δ in Unknown Peaks of High Performance Liquid Chromatography of Hemoglobin in β-Thalassemias |
| title_full |
Quadrupole-Time-of-Flight Mass Spectrometric Identification of Hemoglobin Subunits α, β, γ and δ in Unknown Peaks of High Performance Liquid Chromatography of Hemoglobin in β-Thalassemias |
| title_fullStr |
Quadrupole-Time-of-Flight Mass Spectrometric Identification of Hemoglobin Subunits α, β, γ and δ in Unknown Peaks of High Performance Liquid Chromatography of Hemoglobin in β-Thalassemias |
| title_full_unstemmed |
Quadrupole-Time-of-Flight Mass Spectrometric Identification of Hemoglobin Subunits α, β, γ and δ in Unknown Peaks of High Performance Liquid Chromatography of Hemoglobin in β-Thalassemias |
| title_sort |
Quadrupole-Time-of-Flight Mass Spectrometric Identification of Hemoglobin Subunits α, β, γ and δ in Unknown Peaks of High Performance Liquid Chromatography of Hemoglobin in β-Thalassemias |
| publishDate |
2019 |
| container_title |
Hemoglobin |
| container_volume |
43 |
| container_issue |
3 |
| doi_str_mv |
10.1080/03630269.2019.1632893 |
| url |
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85068914289&doi=10.1080%2f03630269.2019.1632893&partnerID=40&md5=d0a666ecbe676cf7ea5f3b27e81a67ef |
| description |
This is the first report of quadrupole time-of-flight (Q-TOF) mass spectrometric identification of the hemoglobin (Hb) subunits, α, β, δ and γ peptides, derived from enzymatic-digestion of proteins in the early unknown peaks of the cation exchange chromatography of Hb. The objectives were to identify the unknown high performance liquid chromatography (HPLC) peaks in healthy subjects and in patients with β-thalassemia (β-thal). The results demonstrate the existence of pools of free globin chains in red blood cells (RBCs). The α-, β-, δ- and γ-globin peptides were identified in the unknown HPLC peaks. The quantification and role of the free globin pool in patients with β-thal requires further investigation. Identification of all types of Hb subunits in the retention time (RT) before 1 min. suggests that altered Hbs is the nature of these fast-eluting peaks. Relevancy of thalassemias to the protein-aggregation disorders will require review of the role of free globin in the pathology of the disease. © 2019, © 2019 Informa UK Limited, trading as Taylor & Francis Group. |
| publisher |
Taylor and Francis Ltd |
| issn |
3630269 |
| language |
English |
| format |
Article |
| accesstype |
|
| record_format |
scopus |
| collection |
Scopus |
| _version_ |
1818940562335596544 |