Liquid Chromatography Mass Spectrometry (LC-MS) analysis in determining the saliva protein of orthodontic patients during retention phase

• Published in: Journal of Clinical and Experimental Dentistry
• Main Author: Awang-Kechik N.-H.; Ahmad R.; Doustjalali S.-R.; Sabet N.-S.; Abd-Rahman A.-N.-A.
• Format: Article
• Language: English
• Published: Medicina Oral S.L. 2019
• Online Access: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85064045390&doi=10.4317%2fjced.55546&partnerID=40&md5=d38ba4b05ca3d328ea09b20ff8a805ca

Background: The biological responses involved during retention phase have been studied for many years but little is known about the effect of saliva proteome during retention phase of post-orthodontic treatment. This study aims to identify the protein profiles during retention phase in relation to biological processes involved by Liquid Chromatography Mass Spectrometry (LC-MS) approach. Material and Methods: A total of 5 ml of unstimulated saliva was collected from each subject (10 non-orthodontic patients and 15 post-orthodontic patients with 6-months retention phase). Samples were then subjected to LC-MS analysis. The expressed proteins were identified and compared between groups. Incisor irregularity for both maxilla and mandible were determined with Little's Irregularity Index at 6-months retention phase. Results: 146 proteins and 135 proteins were expressed in control and 6-months retention phase group respectively. 15 proteins were identified to be co-expressed between groups. Immune system process was only detected in 6-months retention phase group. Detected protein in immune system process was identified as Tyrosine-protein kinase Tec. Statistical significant of incisor irregularity was only found in mandible at 6-months retention phase. Conclusions: Our study suggests that immune system process protein which is Tyrosine-protein kinase Tec could be used as biomarker for prediction of stability during retention phase of post-orthodontic treatment. © Medicina Oral S. L. C.I.F. B 96689336 - eISSN: 1989-5488.