Bioactive 2-(methyldithio)pyridine-3-carbonitrile from Persian shallot (allium stipitatum regel.) exerts broad-spectrum antimicrobial activity
Antibiotic resistance is a problem that continues to challenge the healthcare sector, especially in clinically significant pathogens like methicillin-resistant Staphylococcus aureus (MRSA). Herein is described the isolation and structure elucidation of a bioactive compound from Allium stipitatum wit...
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MDPI AG
2019
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Online Access: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85062954304&doi=10.3390%2fmolecules24061003&partnerID=40&md5=c9511de441414f04f47951087bfc13f6 |
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2-s2.0-85062954304 Karunanidhi A.; Ghaznavi-Rad E.; Nathan J.J.; Joseph N.; Chigurupati S.; Fauzi F.M.; Pichika M.R.; Hamat R.A.; Lung L.T.T.; Van Belkum A.; Neela V. Bioactive 2-(methyldithio)pyridine-3-carbonitrile from Persian shallot (allium stipitatum regel.) exerts broad-spectrum antimicrobial activity 2019 Molecules 24 6 10.3390/molecules24061003 https://www.scopus.com/inward/record.uri?eid=2-s2.0-85062954304&doi=10.3390%2fmolecules24061003&partnerID=40&md5=c9511de441414f04f47951087bfc13f6 Antibiotic resistance is a problem that continues to challenge the healthcare sector, especially in clinically significant pathogens like methicillin-resistant Staphylococcus aureus (MRSA). Herein is described the isolation and structure elucidation of a bioactive compound from Allium stipitatum with antimicrobial activity. Crude Allium stipitatum dichloromethane extract (ASDE) was subjected to systematic purification by chromatographic procedures to afford various bioactive fractions. A fraction that exhibited anti-MRSA activity (4 µg·mL−1) was further characterized to determine the structure. The structure of the compound was elucidated as 2-(methyldithio)pyridine-3-carbonitrile (2-Medpy-3-CN). The 2-Medpy-3-CN compound, which was screened for antimicrobial activity, exhibited minimum inhibitory concentrations (MICs) in the range of 0.5 to >64 µg·mL−1 for tested bacterial species and 0.25 to 2 µg·mL−1 for Candida spp. Further studies are important to confirm the drug target and mechanism of action. © 2019 by the authors. MDPI AG 14203049 English Article All Open Access; Gold Open Access; Green Open Access |
author |
Karunanidhi A.; Ghaznavi-Rad E.; Nathan J.J.; Joseph N.; Chigurupati S.; Fauzi F.M.; Pichika M.R.; Hamat R.A.; Lung L.T.T.; Van Belkum A.; Neela V. |
spellingShingle |
Karunanidhi A.; Ghaznavi-Rad E.; Nathan J.J.; Joseph N.; Chigurupati S.; Fauzi F.M.; Pichika M.R.; Hamat R.A.; Lung L.T.T.; Van Belkum A.; Neela V. Bioactive 2-(methyldithio)pyridine-3-carbonitrile from Persian shallot (allium stipitatum regel.) exerts broad-spectrum antimicrobial activity |
author_facet |
Karunanidhi A.; Ghaznavi-Rad E.; Nathan J.J.; Joseph N.; Chigurupati S.; Fauzi F.M.; Pichika M.R.; Hamat R.A.; Lung L.T.T.; Van Belkum A.; Neela V. |
author_sort |
Karunanidhi A.; Ghaznavi-Rad E.; Nathan J.J.; Joseph N.; Chigurupati S.; Fauzi F.M.; Pichika M.R.; Hamat R.A.; Lung L.T.T.; Van Belkum A.; Neela V. |
title |
Bioactive 2-(methyldithio)pyridine-3-carbonitrile from Persian shallot (allium stipitatum regel.) exerts broad-spectrum antimicrobial activity |
title_short |
Bioactive 2-(methyldithio)pyridine-3-carbonitrile from Persian shallot (allium stipitatum regel.) exerts broad-spectrum antimicrobial activity |
title_full |
Bioactive 2-(methyldithio)pyridine-3-carbonitrile from Persian shallot (allium stipitatum regel.) exerts broad-spectrum antimicrobial activity |
title_fullStr |
Bioactive 2-(methyldithio)pyridine-3-carbonitrile from Persian shallot (allium stipitatum regel.) exerts broad-spectrum antimicrobial activity |
title_full_unstemmed |
Bioactive 2-(methyldithio)pyridine-3-carbonitrile from Persian shallot (allium stipitatum regel.) exerts broad-spectrum antimicrobial activity |
title_sort |
Bioactive 2-(methyldithio)pyridine-3-carbonitrile from Persian shallot (allium stipitatum regel.) exerts broad-spectrum antimicrobial activity |
publishDate |
2019 |
container_title |
Molecules |
container_volume |
24 |
container_issue |
6 |
doi_str_mv |
10.3390/molecules24061003 |
url |
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85062954304&doi=10.3390%2fmolecules24061003&partnerID=40&md5=c9511de441414f04f47951087bfc13f6 |
description |
Antibiotic resistance is a problem that continues to challenge the healthcare sector, especially in clinically significant pathogens like methicillin-resistant Staphylococcus aureus (MRSA). Herein is described the isolation and structure elucidation of a bioactive compound from Allium stipitatum with antimicrobial activity. Crude Allium stipitatum dichloromethane extract (ASDE) was subjected to systematic purification by chromatographic procedures to afford various bioactive fractions. A fraction that exhibited anti-MRSA activity (4 µg·mL−1) was further characterized to determine the structure. The structure of the compound was elucidated as 2-(methyldithio)pyridine-3-carbonitrile (2-Medpy-3-CN). The 2-Medpy-3-CN compound, which was screened for antimicrobial activity, exhibited minimum inhibitory concentrations (MICs) in the range of 0.5 to >64 µg·mL−1 for tested bacterial species and 0.25 to 2 µg·mL−1 for Candida spp. Further studies are important to confirm the drug target and mechanism of action. © 2019 by the authors. |
publisher |
MDPI AG |
issn |
14203049 |
language |
English |
format |
Article |
accesstype |
All Open Access; Gold Open Access; Green Open Access |
record_format |
scopus |
collection |
Scopus |
_version_ |
1820775469669154816 |