Bioactive 2-(methyldithio)pyridine-3-carbonitrile from Persian shallot (allium stipitatum regel.) exerts broad-spectrum antimicrobial activity

Antibiotic resistance is a problem that continues to challenge the healthcare sector, especially in clinically significant pathogens like methicillin-resistant Staphylococcus aureus (MRSA). Herein is described the isolation and structure elucidation of a bioactive compound from Allium stipitatum wit...

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Published in:Molecules
Main Author: Karunanidhi A.; Ghaznavi-Rad E.; Nathan J.J.; Joseph N.; Chigurupati S.; Fauzi F.M.; Pichika M.R.; Hamat R.A.; Lung L.T.T.; Van Belkum A.; Neela V.
Format: Article
Language:English
Published: MDPI AG 2019
Online Access:https://www.scopus.com/inward/record.uri?eid=2-s2.0-85062954304&doi=10.3390%2fmolecules24061003&partnerID=40&md5=c9511de441414f04f47951087bfc13f6
id 2-s2.0-85062954304
spelling 2-s2.0-85062954304
Karunanidhi A.; Ghaznavi-Rad E.; Nathan J.J.; Joseph N.; Chigurupati S.; Fauzi F.M.; Pichika M.R.; Hamat R.A.; Lung L.T.T.; Van Belkum A.; Neela V.
Bioactive 2-(methyldithio)pyridine-3-carbonitrile from Persian shallot (allium stipitatum regel.) exerts broad-spectrum antimicrobial activity
2019
Molecules
24
6
10.3390/molecules24061003
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85062954304&doi=10.3390%2fmolecules24061003&partnerID=40&md5=c9511de441414f04f47951087bfc13f6
Antibiotic resistance is a problem that continues to challenge the healthcare sector, especially in clinically significant pathogens like methicillin-resistant Staphylococcus aureus (MRSA). Herein is described the isolation and structure elucidation of a bioactive compound from Allium stipitatum with antimicrobial activity. Crude Allium stipitatum dichloromethane extract (ASDE) was subjected to systematic purification by chromatographic procedures to afford various bioactive fractions. A fraction that exhibited anti-MRSA activity (4 µg·mL−1) was further characterized to determine the structure. The structure of the compound was elucidated as 2-(methyldithio)pyridine-3-carbonitrile (2-Medpy-3-CN). The 2-Medpy-3-CN compound, which was screened for antimicrobial activity, exhibited minimum inhibitory concentrations (MICs) in the range of 0.5 to >64 µg·mL−1 for tested bacterial species and 0.25 to 2 µg·mL−1 for Candida spp. Further studies are important to confirm the drug target and mechanism of action. © 2019 by the authors.
MDPI AG
14203049
English
Article
All Open Access; Gold Open Access; Green Open Access
author Karunanidhi A.; Ghaznavi-Rad E.; Nathan J.J.; Joseph N.; Chigurupati S.; Fauzi F.M.; Pichika M.R.; Hamat R.A.; Lung L.T.T.; Van Belkum A.; Neela V.
spellingShingle Karunanidhi A.; Ghaznavi-Rad E.; Nathan J.J.; Joseph N.; Chigurupati S.; Fauzi F.M.; Pichika M.R.; Hamat R.A.; Lung L.T.T.; Van Belkum A.; Neela V.
Bioactive 2-(methyldithio)pyridine-3-carbonitrile from Persian shallot (allium stipitatum regel.) exerts broad-spectrum antimicrobial activity
author_facet Karunanidhi A.; Ghaznavi-Rad E.; Nathan J.J.; Joseph N.; Chigurupati S.; Fauzi F.M.; Pichika M.R.; Hamat R.A.; Lung L.T.T.; Van Belkum A.; Neela V.
author_sort Karunanidhi A.; Ghaznavi-Rad E.; Nathan J.J.; Joseph N.; Chigurupati S.; Fauzi F.M.; Pichika M.R.; Hamat R.A.; Lung L.T.T.; Van Belkum A.; Neela V.
title Bioactive 2-(methyldithio)pyridine-3-carbonitrile from Persian shallot (allium stipitatum regel.) exerts broad-spectrum antimicrobial activity
title_short Bioactive 2-(methyldithio)pyridine-3-carbonitrile from Persian shallot (allium stipitatum regel.) exerts broad-spectrum antimicrobial activity
title_full Bioactive 2-(methyldithio)pyridine-3-carbonitrile from Persian shallot (allium stipitatum regel.) exerts broad-spectrum antimicrobial activity
title_fullStr Bioactive 2-(methyldithio)pyridine-3-carbonitrile from Persian shallot (allium stipitatum regel.) exerts broad-spectrum antimicrobial activity
title_full_unstemmed Bioactive 2-(methyldithio)pyridine-3-carbonitrile from Persian shallot (allium stipitatum regel.) exerts broad-spectrum antimicrobial activity
title_sort Bioactive 2-(methyldithio)pyridine-3-carbonitrile from Persian shallot (allium stipitatum regel.) exerts broad-spectrum antimicrobial activity
publishDate 2019
container_title Molecules
container_volume 24
container_issue 6
doi_str_mv 10.3390/molecules24061003
url https://www.scopus.com/inward/record.uri?eid=2-s2.0-85062954304&doi=10.3390%2fmolecules24061003&partnerID=40&md5=c9511de441414f04f47951087bfc13f6
description Antibiotic resistance is a problem that continues to challenge the healthcare sector, especially in clinically significant pathogens like methicillin-resistant Staphylococcus aureus (MRSA). Herein is described the isolation and structure elucidation of a bioactive compound from Allium stipitatum with antimicrobial activity. Crude Allium stipitatum dichloromethane extract (ASDE) was subjected to systematic purification by chromatographic procedures to afford various bioactive fractions. A fraction that exhibited anti-MRSA activity (4 µg·mL−1) was further characterized to determine the structure. The structure of the compound was elucidated as 2-(methyldithio)pyridine-3-carbonitrile (2-Medpy-3-CN). The 2-Medpy-3-CN compound, which was screened for antimicrobial activity, exhibited minimum inhibitory concentrations (MICs) in the range of 0.5 to >64 µg·mL−1 for tested bacterial species and 0.25 to 2 µg·mL−1 for Candida spp. Further studies are important to confirm the drug target and mechanism of action. © 2019 by the authors.
publisher MDPI AG
issn 14203049
language English
format Article
accesstype All Open Access; Gold Open Access; Green Open Access
record_format scopus
collection Scopus
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