Enhancement and optimization of sinensetin extract from orthosiphon stamineus using supercrtitical carbon dioxide extraction; [Peningkatan dan pengoptimuman ekstrak sinensetin dari orthosiphon stamineus menggunakan kaedah pengekstrakan bendalir supergenting karbon dioksida]
Sinensetin is a rare valuable flavonoid compound that contains 5 methoxy groups attached on the flavone backbone. This compound is abundantly established in citrus peel and can also be found in medicinal herb, ‘misai kucing’. Scientifically, ‘misai kucing’ is known as Orthosiphon stamineus; mostly u...
Published in: | Malaysian Journal of Analytical Sciences |
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Malaysian Society of Analytical Sciences
2018
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2-s2.0-85057157173 Aziz A.H.A.; Yunus M.A.C.; Yian L.N.; Idham Z.; Rithwan F.; Hadzri H.M.; Mustapha A.N. Enhancement and optimization of sinensetin extract from orthosiphon stamineus using supercrtitical carbon dioxide extraction; [Peningkatan dan pengoptimuman ekstrak sinensetin dari orthosiphon stamineus menggunakan kaedah pengekstrakan bendalir supergenting karbon dioksida] 2018 Malaysian Journal of Analytical Sciences 22 5 10.17576/mjas-2018-2205-14 https://www.scopus.com/inward/record.uri?eid=2-s2.0-85057157173&doi=10.17576%2fmjas-2018-2205-14&partnerID=40&md5=3b3eaee525e9648e6432449b19f3fa41 Sinensetin is a rare valuable flavonoid compound that contains 5 methoxy groups attached on the flavone backbone. This compound is abundantly established in citrus peel and can also be found in medicinal herb, ‘misai kucing’. Scientifically, ‘misai kucing’ is known as Orthosiphon stamineus; mostly used to treat kidney stones by old folks. Orthosiphon stamineus was listed in National Key Economic Area (NKEA) as contributor towards the Malaysian Economic Growth. Thus, this study was conducted with objectives to determine suitable modifier for supercritical carbon dioxide (SC-CO2) extraction and to optimize the SC-CO2 extraction condition (pressure, temperature and average particle size) on the concentration of sinensetin. Water, methanol and acetone were used on the determination of modifier for SC-CO2 extraction by using water bath extraction at 40 °C in 8 hours. The results showed that acetone gave the highest percentage of sinensetin extract of 0.11%. Therefore, acetone was used as a modifier to enhance the extraction of sinensetin. The concentration of sinensetin was increased and the highest percentage of sinensetin obtained was 6.99%. Response surface methodology (RSM) through Box-Behnken design via Design Expert Software was applied to optimize the SC-CO2 conditions. The first-order polynomial was used to express the percentage of sinensetin with R2 value of 0.542. The optimum SC-CO2 operating condition was 30 MPa, 40 °C and 308.24 µm with sinensetin percentage of 4.35%. Hence, SC-CO2 with small amount of modifier was the best method to extract sinensetin as compared to water bath extraction method. © 2018, Malaysian Society of Analytical Sciences. All rights reserved. Malaysian Society of Analytical Sciences 13942506 English Article All Open Access; Gold Open Access |
author |
Aziz A.H.A.; Yunus M.A.C.; Yian L.N.; Idham Z.; Rithwan F.; Hadzri H.M.; Mustapha A.N. |
spellingShingle |
Aziz A.H.A.; Yunus M.A.C.; Yian L.N.; Idham Z.; Rithwan F.; Hadzri H.M.; Mustapha A.N. Enhancement and optimization of sinensetin extract from orthosiphon stamineus using supercrtitical carbon dioxide extraction; [Peningkatan dan pengoptimuman ekstrak sinensetin dari orthosiphon stamineus menggunakan kaedah pengekstrakan bendalir supergenting karbon dioksida] |
author_facet |
Aziz A.H.A.; Yunus M.A.C.; Yian L.N.; Idham Z.; Rithwan F.; Hadzri H.M.; Mustapha A.N. |
author_sort |
Aziz A.H.A.; Yunus M.A.C.; Yian L.N.; Idham Z.; Rithwan F.; Hadzri H.M.; Mustapha A.N. |
title |
Enhancement and optimization of sinensetin extract from orthosiphon stamineus using supercrtitical carbon dioxide extraction; [Peningkatan dan pengoptimuman ekstrak sinensetin dari orthosiphon stamineus menggunakan kaedah pengekstrakan bendalir supergenting karbon dioksida] |
title_short |
Enhancement and optimization of sinensetin extract from orthosiphon stamineus using supercrtitical carbon dioxide extraction; [Peningkatan dan pengoptimuman ekstrak sinensetin dari orthosiphon stamineus menggunakan kaedah pengekstrakan bendalir supergenting karbon dioksida] |
title_full |
Enhancement and optimization of sinensetin extract from orthosiphon stamineus using supercrtitical carbon dioxide extraction; [Peningkatan dan pengoptimuman ekstrak sinensetin dari orthosiphon stamineus menggunakan kaedah pengekstrakan bendalir supergenting karbon dioksida] |
title_fullStr |
Enhancement and optimization of sinensetin extract from orthosiphon stamineus using supercrtitical carbon dioxide extraction; [Peningkatan dan pengoptimuman ekstrak sinensetin dari orthosiphon stamineus menggunakan kaedah pengekstrakan bendalir supergenting karbon dioksida] |
title_full_unstemmed |
Enhancement and optimization of sinensetin extract from orthosiphon stamineus using supercrtitical carbon dioxide extraction; [Peningkatan dan pengoptimuman ekstrak sinensetin dari orthosiphon stamineus menggunakan kaedah pengekstrakan bendalir supergenting karbon dioksida] |
title_sort |
Enhancement and optimization of sinensetin extract from orthosiphon stamineus using supercrtitical carbon dioxide extraction; [Peningkatan dan pengoptimuman ekstrak sinensetin dari orthosiphon stamineus menggunakan kaedah pengekstrakan bendalir supergenting karbon dioksida] |
publishDate |
2018 |
container_title |
Malaysian Journal of Analytical Sciences |
container_volume |
22 |
container_issue |
5 |
doi_str_mv |
10.17576/mjas-2018-2205-14 |
url |
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85057157173&doi=10.17576%2fmjas-2018-2205-14&partnerID=40&md5=3b3eaee525e9648e6432449b19f3fa41 |
description |
Sinensetin is a rare valuable flavonoid compound that contains 5 methoxy groups attached on the flavone backbone. This compound is abundantly established in citrus peel and can also be found in medicinal herb, ‘misai kucing’. Scientifically, ‘misai kucing’ is known as Orthosiphon stamineus; mostly used to treat kidney stones by old folks. Orthosiphon stamineus was listed in National Key Economic Area (NKEA) as contributor towards the Malaysian Economic Growth. Thus, this study was conducted with objectives to determine suitable modifier for supercritical carbon dioxide (SC-CO2) extraction and to optimize the SC-CO2 extraction condition (pressure, temperature and average particle size) on the concentration of sinensetin. Water, methanol and acetone were used on the determination of modifier for SC-CO2 extraction by using water bath extraction at 40 °C in 8 hours. The results showed that acetone gave the highest percentage of sinensetin extract of 0.11%. Therefore, acetone was used as a modifier to enhance the extraction of sinensetin. The concentration of sinensetin was increased and the highest percentage of sinensetin obtained was 6.99%. Response surface methodology (RSM) through Box-Behnken design via Design Expert Software was applied to optimize the SC-CO2 conditions. The first-order polynomial was used to express the percentage of sinensetin with R2 value of 0.542. The optimum SC-CO2 operating condition was 30 MPa, 40 °C and 308.24 µm with sinensetin percentage of 4.35%. Hence, SC-CO2 with small amount of modifier was the best method to extract sinensetin as compared to water bath extraction method. © 2018, Malaysian Society of Analytical Sciences. All rights reserved. |
publisher |
Malaysian Society of Analytical Sciences |
issn |
13942506 |
language |
English |
format |
Article |
accesstype |
All Open Access; Gold Open Access |
record_format |
scopus |
collection |
Scopus |
_version_ |
1809677907040141312 |