Size and time-dependent induction of proinflammatory cytokines expression in brains of mice treated with gold nanoparticles

Gold nanoparticles (GNPs) are among the ideal nano-sized materials for medical applications such as imaging and drug delivery. Considering the significance of recent reports on acute phase induction of inflammatory mediators by GNPs, we studied the effect of GNPs on proinflammatory cytokines gene ex...

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Published in:Saudi Journal of Biological Sciences
Main Author: Khan H.A.; Alamery S.; Ibrahim K.E.; El-Nagar D.M.; Al-Harbi N.; Rusop M.; Alrokayan S.H.
Format: Article
Language:English
Published: Elsevier B.V. 2019
Online Access:https://www.scopus.com/inward/record.uri?eid=2-s2.0-85054179478&doi=10.1016%2fj.sjbs.2018.09.012&partnerID=40&md5=ef07c1142772d63564f8ab46a3fa9140
id 2-s2.0-85054179478
spelling 2-s2.0-85054179478
Khan H.A.; Alamery S.; Ibrahim K.E.; El-Nagar D.M.; Al-Harbi N.; Rusop M.; Alrokayan S.H.
Size and time-dependent induction of proinflammatory cytokines expression in brains of mice treated with gold nanoparticles
2019
Saudi Journal of Biological Sciences
26
3
10.1016/j.sjbs.2018.09.012
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85054179478&doi=10.1016%2fj.sjbs.2018.09.012&partnerID=40&md5=ef07c1142772d63564f8ab46a3fa9140
Gold nanoparticles (GNPs) are among the ideal nano-sized materials for medical applications such as imaging and drug delivery. Considering the significance of recent reports on acute phase induction of inflammatory mediators by GNPs, we studied the effect of GNPs on proinflammatory cytokines gene expression in mouse brain. Group 1 served as control whereas groups 2–4 were given only one intraperitoneal dose of 5, 20 and 50 nm GNPs, respectively and sacrificed after 24 h. The animals in groups 5–7 also received the same treatment but sacrificed after 7 days. Groups 8–10 received two injections of GNPs (5, 20 and 50 nm, respectively), first at the beginning of study and second on day 6, and sacrificed on day 7. Total RNA was extracted from the cerebral tissue and analyzed for the gene expressions of IL-1β, IL-6 and TNF-α. A single injection of 5 nm diameter GNPs significantly increased the mRNA expression of IL-1β and IL-6 in mouse brain on day 7, which was not augmented by the second dose of the same GNPs. Larger size GNPs (20 nm and 50 nm) did not cause any significant change in the expression of proinflammatory cytokines in mouse brain. In conclusion, systemic administration of small sized GNPs (5 nm) induced a proinflammatory cascade in mouse brain indicating a crucial role of GNPs size on immune response. It is important to use the right sized GNPs in order to avoid an acute phase inflammatory response that could be cytotoxic or interfere with the bioavailability of nanomaterials. © 2018 The Authors
Elsevier B.V.
1319562X
English
Article
All Open Access; Hybrid Gold Open Access
author Khan H.A.; Alamery S.; Ibrahim K.E.; El-Nagar D.M.; Al-Harbi N.; Rusop M.; Alrokayan S.H.
spellingShingle Khan H.A.; Alamery S.; Ibrahim K.E.; El-Nagar D.M.; Al-Harbi N.; Rusop M.; Alrokayan S.H.
Size and time-dependent induction of proinflammatory cytokines expression in brains of mice treated with gold nanoparticles
author_facet Khan H.A.; Alamery S.; Ibrahim K.E.; El-Nagar D.M.; Al-Harbi N.; Rusop M.; Alrokayan S.H.
author_sort Khan H.A.; Alamery S.; Ibrahim K.E.; El-Nagar D.M.; Al-Harbi N.; Rusop M.; Alrokayan S.H.
title Size and time-dependent induction of proinflammatory cytokines expression in brains of mice treated with gold nanoparticles
title_short Size and time-dependent induction of proinflammatory cytokines expression in brains of mice treated with gold nanoparticles
title_full Size and time-dependent induction of proinflammatory cytokines expression in brains of mice treated with gold nanoparticles
title_fullStr Size and time-dependent induction of proinflammatory cytokines expression in brains of mice treated with gold nanoparticles
title_full_unstemmed Size and time-dependent induction of proinflammatory cytokines expression in brains of mice treated with gold nanoparticles
title_sort Size and time-dependent induction of proinflammatory cytokines expression in brains of mice treated with gold nanoparticles
publishDate 2019
container_title Saudi Journal of Biological Sciences
container_volume 26
container_issue 3
doi_str_mv 10.1016/j.sjbs.2018.09.012
url https://www.scopus.com/inward/record.uri?eid=2-s2.0-85054179478&doi=10.1016%2fj.sjbs.2018.09.012&partnerID=40&md5=ef07c1142772d63564f8ab46a3fa9140
description Gold nanoparticles (GNPs) are among the ideal nano-sized materials for medical applications such as imaging and drug delivery. Considering the significance of recent reports on acute phase induction of inflammatory mediators by GNPs, we studied the effect of GNPs on proinflammatory cytokines gene expression in mouse brain. Group 1 served as control whereas groups 2–4 were given only one intraperitoneal dose of 5, 20 and 50 nm GNPs, respectively and sacrificed after 24 h. The animals in groups 5–7 also received the same treatment but sacrificed after 7 days. Groups 8–10 received two injections of GNPs (5, 20 and 50 nm, respectively), first at the beginning of study and second on day 6, and sacrificed on day 7. Total RNA was extracted from the cerebral tissue and analyzed for the gene expressions of IL-1β, IL-6 and TNF-α. A single injection of 5 nm diameter GNPs significantly increased the mRNA expression of IL-1β and IL-6 in mouse brain on day 7, which was not augmented by the second dose of the same GNPs. Larger size GNPs (20 nm and 50 nm) did not cause any significant change in the expression of proinflammatory cytokines in mouse brain. In conclusion, systemic administration of small sized GNPs (5 nm) induced a proinflammatory cascade in mouse brain indicating a crucial role of GNPs size on immune response. It is important to use the right sized GNPs in order to avoid an acute phase inflammatory response that could be cytotoxic or interfere with the bioavailability of nanomaterials. © 2018 The Authors
publisher Elsevier B.V.
issn 1319562X
language English
format Article
accesstype All Open Access; Hybrid Gold Open Access
record_format scopus
collection Scopus
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