Correlation Study of PVDF Membrane Morphology with Protein Adsorption: Quantitative Analysis by FTIR/ATR Technique

• Published in: IOP Conference Series: Materials Science and Engineering
• Main Author: Ideris N.; Ahmad A.L.; Ooi B.S.; Low S.C.
• Format: Conference paper
• Language: English
• Published: Institute of Physics Publishing 2018
• Online Access: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85050609948&doi=10.1088%2f1757-899X%2f358%2f1%2f012054&partnerID=40&md5=0e4c6fb13d4bb734da6e249fab389a9f

Microporous PVDF membranes were used as protein capture matrices in immunoassays. Because the most common labels in immunoassays were detected based on the colour change, an understanding of how protein concentration varies on different PVDF surfaces was needed. Herein, the correlation between the membrane pore size and protein adsorption was systematically investigated. Five different PVDF membrane morphologies were prepared and FTIR/ATR was employed to accurately quantify the surface protein concentration on membranes with small pore sizes. SigmaPlot® was used to find a suitable curve fit for protein adsorption and membrane pore size, with a high correlation coefficient, R2, of 0.9971. © Published under licence by IOP Publishing Ltd.