Storage effect of air liur sample on quality of genomic DNA; [Kesan Penyimpanan Sampel Air Liur Terhadap Kualiti DNA Genom]

• Published in: Sains Malaysiana
• Main Author: Wahab R.M.A.; Nasri F.A.M.; Abidin I.Z.Z.; Ariffin Z.Z.; Yazid M.D.; Ariffin S.H.Z.
• Format: Article
• Language: Malay
• Published: Penerbit Universiti Kebangsaan Malaysia 2017
• Online Access: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85029459000&doi=10.17576%2fjsm-2017-4606-10&partnerID=40&md5=d18386af48b953650edaf9b55079dd8e

Air liur is a potential source of DNA easily obtained for clinical studies because it is non-invasive compared to blood samples. This study was carried out to isolate and purify genomic DNA from human saliva sample and to study storage effect on the quality of genomic DNA. Air liur samples (n=5) were kept in Tris-NaCl EDTA (TNE) buffer at room temperature (25°C) according to fixed time period which are; fresh (without storage), 1, 2, 3 and 4 months. Isolation and purification of DNA was carried out using phenol-chloroform method. Next, PCR was conducted to determine the purity of extracted DNA by using amplification of beta-globin sequence region and identify bacterial existence using the sequence that codes for 16S rDNA. Only human beta-globin genomic DNA fragment was successfully amplified from fresh sample. Air liur sample kept in TNE buffer at room temperature was not able to stabilize human genomic DNA at long term and worked for short term storage which was less than a month. In conclusion, fresh saliva sample is needed to isolate genomic DNA.