| Summary: | One standard chromatographic conditions of HPLC was developed for different Dipterocarpaceae plant extracts. The purpose of this study is to achieve an optimized chromatographic method that is able to produce a reasonably good separation and resolution of oligostilbenes in plant crude extracts. For this purpose, five model samples of Dipterocarpaceae extracts, Shorea parvifolia, Shorea ovalis, Dipterocarpus crinitus, Dipterocarpus gracilis and Hopea spp. were used. The parameters, which include types of chromatographic solvents, types of mobile phase elution, solvent composition and the injection volumes, were modified. The results showed that the optimum chromatographic conditions were achieved when a gradient elution was adopted, with the solvent composition of H2O: ACN from 95:5 to 25:75 in 20 minutes. The injection volume of 5 μL is best for a 1 mg/ml sample concentration. The temperature was maintained at 35 °C and the flow rate was at 1.0 ml/min. Some compromises were taken into account such as having less accurate method if compared with a single method developed for single extract or sample. The method showed positive results when tested on 10 extracts of the same family from different plant parts and localities. © 2017, Malaysian Society of Analytical Sciences. All rights reserved.
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