Plasmid profile analysis of clinical isolates of acinetobacter species in Malaysia

• Published in: Research Journal of Pharmaceutical, Biological and Chemical Sciences
• Main Author: Nazmul M.H.M.; Jamal H.; Rashid M.A.; Al-Jashamy K.; Doustjalali S.R.; Sabet N.S.
• Format: Article
• Language: English
• Published: Research Journal of Pharmaceutical, Biological and Chemical Sciences 2016
• Online Access: https://www.scopus.com/inward/record.uri?eid=2-s2.0-84996619202&partnerID=40&md5=1c42a74551eb921fa1acab8c33f8b717

Forty clinical isolates of Acinetobacter species were collected from Selayang hospital, Selangor, Malaysia. The strains were isolated from respiratory tract, urine and pus. All the isolates were re-identified and confirmed as Acinetobacter species in our laboratory. Plasmids were detected in 16 isolates with the plasmid occurrence rate (POR) of 40%. These 16 isolates appear to harbor 1 or more plasmids with the maximum of 4 plasmids. The sizes of the plasmid DNA range from the lowest 1.8 kb to the highest 8 kb. Supercoil DNA marker was used to provide reference plasmids of known molecular weight. This helped in calculating the molecular weight of the plasmids in this study.