Prevalence of verotoxin gene among the clinical isolates of non-O157 diarrheagenic Escherichia coli in Malaysia

Prevalence of verotoxin gene among the clinical isolates of non-O157 diarrheagenic Escherichia coli in Malaysia

A total of 48 non-O157 diarrheagenic Escherichia coli isolated from General Hospital Kuala Lumpur, Malaysia, from children with diarrhea were examined. This study proved the polymerase chain reaction used to detect verotoxin (VT) gene was sensitive, specific, rapid and reproducible. Two sets of prim...

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Published in:Research Journal of Pharmaceutical, Biological and Chemical Sciences
Main Author: Nazmul M.H.M.; Jamal H.; Rashid M.A.; Al-Jashamy K.; Doustjalali S.R.; Sabet N.S.
Format: Article
Language:English
Published: Research Journal of Pharmaceutical, Biological and Chemical Sciences 2016
Online Access:https://www.scopus.com/inward/record.uri?eid=2-s2.0-84996486793&partnerID=40&md5=94de1ecb3c069e2a5ae89148ad79ead5
id 2-s2.0-84996486793
spelling 2-s2.0-84996486793
Nazmul M.H.M.; Jamal H.; Rashid M.A.; Al-Jashamy K.; Doustjalali S.R.; Sabet N.S.
Prevalence of verotoxin gene among the clinical isolates of non-O157 diarrheagenic Escherichia coli in Malaysia
2016
Research Journal of Pharmaceutical, Biological and Chemical Sciences
7
6

https://www.scopus.com/inward/record.uri?eid=2-s2.0-84996486793&partnerID=40&md5=94de1ecb3c069e2a5ae89148ad79ead5
A total of 48 non-O157 diarrheagenic Escherichia coli isolated from General Hospital Kuala Lumpur, Malaysia, from children with diarrhea were examined. This study proved the polymerase chain reaction used to detect verotoxin (VT) gene was sensitive, specific, rapid and reproducible. Two sets of primers (VT1 and VT2) were used. Both the primers produced a fragment of 348 bp of VT1 gene and 584 bp of VT2 gene, respectively. It was found that 12 E. coli isolates (25%) carried VT1 gene whereas no isolate carried VT2 gene.VT1 was found to be the most common verotoxin among the non-O157 diarrheagenic Escherichia coli strains isolated from clinical sources in Malaysia.
Research Journal of Pharmaceutical, Biological and Chemical Sciences
9758585
English
Article
author Nazmul M.H.M.; Jamal H.; Rashid M.A.; Al-Jashamy K.; Doustjalali S.R.; Sabet N.S.
spellingShingle Nazmul M.H.M.; Jamal H.; Rashid M.A.; Al-Jashamy K.; Doustjalali S.R.; Sabet N.S.
Prevalence of verotoxin gene among the clinical isolates of non-O157 diarrheagenic Escherichia coli in Malaysia
author_facet Nazmul M.H.M.; Jamal H.; Rashid M.A.; Al-Jashamy K.; Doustjalali S.R.; Sabet N.S.
author_sort Nazmul M.H.M.; Jamal H.; Rashid M.A.; Al-Jashamy K.; Doustjalali S.R.; Sabet N.S.
title Prevalence of verotoxin gene among the clinical isolates of non-O157 diarrheagenic Escherichia coli in Malaysia
title_short Prevalence of verotoxin gene among the clinical isolates of non-O157 diarrheagenic Escherichia coli in Malaysia
title_full Prevalence of verotoxin gene among the clinical isolates of non-O157 diarrheagenic Escherichia coli in Malaysia
title_fullStr Prevalence of verotoxin gene among the clinical isolates of non-O157 diarrheagenic Escherichia coli in Malaysia
title_full_unstemmed Prevalence of verotoxin gene among the clinical isolates of non-O157 diarrheagenic Escherichia coli in Malaysia
title_sort Prevalence of verotoxin gene among the clinical isolates of non-O157 diarrheagenic Escherichia coli in Malaysia
publishDate 2016
container_title Research Journal of Pharmaceutical, Biological and Chemical Sciences
container_volume 7
container_issue 6
doi_str_mv
url https://www.scopus.com/inward/record.uri?eid=2-s2.0-84996486793&partnerID=40&md5=94de1ecb3c069e2a5ae89148ad79ead5
description A total of 48 non-O157 diarrheagenic Escherichia coli isolated from General Hospital Kuala Lumpur, Malaysia, from children with diarrhea were examined. This study proved the polymerase chain reaction used to detect verotoxin (VT) gene was sensitive, specific, rapid and reproducible. Two sets of primers (VT1 and VT2) were used. Both the primers produced a fragment of 348 bp of VT1 gene and 584 bp of VT2 gene, respectively. It was found that 12 E. coli isolates (25%) carried VT1 gene whereas no isolate carried VT2 gene.VT1 was found to be the most common verotoxin among the non-O157 diarrheagenic Escherichia coli strains isolated from clinical sources in Malaysia.
publisher Research Journal of Pharmaceutical, Biological and Chemical Sciences
issn 9758585
language English
format Article
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