Prevalence of verotoxin gene among the clinical isolates of non-O157 diarrheagenic Escherichia coli in Malaysia
A total of 48 non-O157 diarrheagenic Escherichia coli isolated from General Hospital Kuala Lumpur, Malaysia, from children with diarrhea were examined. This study proved the polymerase chain reaction used to detect verotoxin (VT) gene was sensitive, specific, rapid and reproducible. Two sets of prim...
| Published in: | Research Journal of Pharmaceutical, Biological and Chemical Sciences |
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| Main Author: | |
| Format: | Article |
| Language: | English |
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Research Journal of Pharmaceutical, Biological and Chemical Sciences
2016
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| Online Access: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84996486793&partnerID=40&md5=94de1ecb3c069e2a5ae89148ad79ead5 |
| Summary: | A total of 48 non-O157 diarrheagenic Escherichia coli isolated from General Hospital Kuala Lumpur, Malaysia, from children with diarrhea were examined. This study proved the polymerase chain reaction used to detect verotoxin (VT) gene was sensitive, specific, rapid and reproducible. Two sets of primers (VT1 and VT2) were used. Both the primers produced a fragment of 348 bp of VT1 gene and 584 bp of VT2 gene, respectively. It was found that 12 E. coli isolates (25%) carried VT1 gene whereas no isolate carried VT2 gene.VT1 was found to be the most common verotoxin among the non-O157 diarrheagenic Escherichia coli strains isolated from clinical sources in Malaysia. |
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| ISSN: | 9758585 |