Supramolecular complexes formed by the self-assembly of hydrophobic bis(Zn2+-cyclen) complexes, copper, and Di- or triimide units for the hydrolysis of phosphate mono- and diesters in two-phase solvent systems (cyclen=1,4,7,10-tetraazacyclododecane)

• Published in: Chemical and Pharmaceutical Bulletin
• Main Author: Hisamatsu Y.; Miyazawa Y.; Yoneda K.; Miyauchi M.; Zulkefeli M.; Aoki S.
• Format: Article
• Language: English
• Published: Pharmaceutical Society of Japan 2016
• Online Access: https://www.scopus.com/inward/record.uri?eid=2-s2.0-84969540653&doi=10.1248%2fcpb.c15-01014&partnerID=40&md5=e51bb248421f062d51eae20e2cbd368f

We previously reported on supramolecular complexes 4 and 5, formed by the 4 : 4 : 4 or 2 : 2 : 2 assembly of a dimeric zinc(II) complex (Zn2L1) having 2,2′-bipyridyl linker, dianion of cyanuric acid (CA) or 5,5-diethylbarbituric acid (Bar), and copper(II) ion (Cu2+) in an aqueous solution. The supermolecule 4 possesses Cu2(μ-OH)2 centers and catalyzes hydrolysis of phosphate monoester dianion, mono(4-nitrophenyl)phosphate (MNP), at neutral pH. In this manuscript, we report on design and synthesis of hydrophobic supermolecules 9 and 10 by 4 : 4 : 4 and 2 : 2 : 2 self-assembly of hydrophobic Zn2L2 and Zn2L3 containing long alkyl chains, CA or Bar, and Cu2+ and their phosphatase activity for the hydrolysis of MNP and bis(4-nitrophenyl)phosphate (BNP) in two-phase solvent systems. We assumed that the Cu2(μ-OH)2 active sites of 9 and 10 would be more stable in organic solvent than in aqueous solution and that product inhibition of the supermolecules might be avoided by the release of HPO42- into the aqueous layer. The findings indicate that 9 and 10 exhibit phosphatase activity in the two-phase solvent system, although catalytic turnover was not observed. Furthermore, the hydrolysis of BNP catalyzed by the hydrophobic 2 : 2 : 2 supermolecules in the two-phase solvent system is described. © 2016 The Pharmaceutical Society of Japan.