Fractionation of hemicellulose from rice straw by alkaline extraction and ethanol precipitation; [Pemisahan hemiselulosa daripada hampas batang padi melalui pengekstrakan beralkali dan pemendakan etanol]

• Published in: Malaysian Journal of Analytical Sciences
• Main Author: Zulyadi N.H.; Saleh S.H.; Sarijo S.H.
• Format: Article
• Language: English
• Published: Malaysian Society of Analytical Sciences 2016
• Online Access: https://www.scopus.com/inward/record.uri?eid=2-s2.0-84964296198&doi=10.17576%2fmjas-2016-2002-15&partnerID=40&md5=5af89fd3d5859a9019dc6873ebdf1bb0
• ISSN: 13942506
• DOI: 10.17576/mjas-2016-2002-15

Hemicelluloses were extracted from rice straw using alkaline extraction and ethanol precipitation. The effects of different extraction conditions; temperature (45-65 °C), pH (4.5-6.5), and concentrations of NaOH (0.25-1 M) on the hemicellulose yield were investigated. Two hemicelllulosic fractions namely Ha1 and Ha2 were obtained by precipitation of alkaline soluble hemicellulose in 0.2 volumes and 4 volumes ethanol, respectively. The Ha1 and Ha2 were further characterized to obtain total sugar, monosaccharides and Klason Lignin content. The highest hemicellulose yield for Ha1 was 4.00% obtained at 55 °C and pH 5.5 with 0.25 M of NaOH, whereas the highest hemicellulose yield for Ha2 was 19.88% obtained at 55 °C and pH 5.5 with 0.5 M of NaOH. This study revealed that hemicellulose yield is dependent on the pH, temperature and concentration of NaOH in the alkaline extraction. © 2016, Malaysian Society of Analytical Sciences. All rights reserved.