Tocotrienol preserves ovarian function in cyclophosphamide therapy

Introduction: Cyclophosphamide (CPA) chemotherapy leads to ovarian failure and infertility. Tocotrienol (T3) is an antioxidant and anti-inflammatory agent. The role of T3 in ovarian protection throughout chemotherapy remains unclear. Aim: To investigate the role of T3 in the preservation of female f...

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Published in:Human and Experimental Toxicology
Main Author: Saleh H.S.; Omar E.; Froemming G.R.A.; Said R.M.
Format: Article
Language:English
Published: SAGE Publications Ltd 2015
Online Access:https://www.scopus.com/inward/record.uri?eid=2-s2.0-84942944444&doi=10.1177%2f0960327114564793&partnerID=40&md5=96b77f144f7106aa6c7234ee59d32a85
id 2-s2.0-84942944444
spelling 2-s2.0-84942944444
Saleh H.S.; Omar E.; Froemming G.R.A.; Said R.M.
Tocotrienol preserves ovarian function in cyclophosphamide therapy
2015
Human and Experimental Toxicology
34
10
10.1177/0960327114564793
https://www.scopus.com/inward/record.uri?eid=2-s2.0-84942944444&doi=10.1177%2f0960327114564793&partnerID=40&md5=96b77f144f7106aa6c7234ee59d32a85
Introduction: Cyclophosphamide (CPA) chemotherapy leads to ovarian failure and infertility. Tocotrienol (T3) is an antioxidant and anti-inflammatory agent. The role of T3 in ovarian protection throughout chemotherapy remains unclear. Aim: To investigate the role of T3 in the preservation of female fertility in CPA treatment. Method: Sixty female mice were divided into five treatment groups, namely, normal saline, corn oil only, T3 only, CPA and CPA + T3. The treatment was given for 30 days, followed by administration of gonadotrophin to induce ovulation. After killing, both ovaries were collected and examined histologically. Results: There was significant reduction in ovarian size in the CPA group compared with the normal group (CPA versus normal, mean area ± SD; 0.118 ± 0.018 vs. 0.423 ± 0.024 cm2; p ‰ 0.005), whilst concurrent administration of T3 with CPA leads to conservation of ovarian size (CPA + T3 vs. CPA, mean area ± SD; 0.285 ± 0.032 vs. 0.118 ± 0.018 cm;be p ‰ 0.005). Ovaries in CPA group showed abnormal folliculogenesis with accompanied reduced ovulation rate, follicular oedema, increased vascularity and inflammatory cell infiltration. These changes were reversed by concurrent T3 administration. Conclusion: Co-administration of T3 with CPA confers protection of ovarian morphology and function in vivo. These findings contribute to the further elucidation of CPA effect on ovary and suggest the potential of T3 use in preserving fertility in chemotherapy. © 2015 SAGE Publications.
SAGE Publications Ltd
09603271
English
Article

author Saleh H.S.; Omar E.; Froemming G.R.A.; Said R.M.
spellingShingle Saleh H.S.; Omar E.; Froemming G.R.A.; Said R.M.
Tocotrienol preserves ovarian function in cyclophosphamide therapy
author_facet Saleh H.S.; Omar E.; Froemming G.R.A.; Said R.M.
author_sort Saleh H.S.; Omar E.; Froemming G.R.A.; Said R.M.
title Tocotrienol preserves ovarian function in cyclophosphamide therapy
title_short Tocotrienol preserves ovarian function in cyclophosphamide therapy
title_full Tocotrienol preserves ovarian function in cyclophosphamide therapy
title_fullStr Tocotrienol preserves ovarian function in cyclophosphamide therapy
title_full_unstemmed Tocotrienol preserves ovarian function in cyclophosphamide therapy
title_sort Tocotrienol preserves ovarian function in cyclophosphamide therapy
publishDate 2015
container_title Human and Experimental Toxicology
container_volume 34
container_issue 10
doi_str_mv 10.1177/0960327114564793
url https://www.scopus.com/inward/record.uri?eid=2-s2.0-84942944444&doi=10.1177%2f0960327114564793&partnerID=40&md5=96b77f144f7106aa6c7234ee59d32a85
description Introduction: Cyclophosphamide (CPA) chemotherapy leads to ovarian failure and infertility. Tocotrienol (T3) is an antioxidant and anti-inflammatory agent. The role of T3 in ovarian protection throughout chemotherapy remains unclear. Aim: To investigate the role of T3 in the preservation of female fertility in CPA treatment. Method: Sixty female mice were divided into five treatment groups, namely, normal saline, corn oil only, T3 only, CPA and CPA + T3. The treatment was given for 30 days, followed by administration of gonadotrophin to induce ovulation. After killing, both ovaries were collected and examined histologically. Results: There was significant reduction in ovarian size in the CPA group compared with the normal group (CPA versus normal, mean area ± SD; 0.118 ± 0.018 vs. 0.423 ± 0.024 cm2; p ‰ 0.005), whilst concurrent administration of T3 with CPA leads to conservation of ovarian size (CPA + T3 vs. CPA, mean area ± SD; 0.285 ± 0.032 vs. 0.118 ± 0.018 cm;be p ‰ 0.005). Ovaries in CPA group showed abnormal folliculogenesis with accompanied reduced ovulation rate, follicular oedema, increased vascularity and inflammatory cell infiltration. These changes were reversed by concurrent T3 administration. Conclusion: Co-administration of T3 with CPA confers protection of ovarian morphology and function in vivo. These findings contribute to the further elucidation of CPA effect on ovary and suggest the potential of T3 use in preserving fertility in chemotherapy. © 2015 SAGE Publications.
publisher SAGE Publications Ltd
issn 09603271
language English
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