Neuroprotective effect from stem bark extracts of Knema laurina against H2O2-and Aβ1-42-induced cell death in human SH-SY5Y cells

• Published in: Natural Product Research
• Main Author: Ismail N.; Akhtar M.N.; Ismail M.; Zareen S.; Shah S.A.A.; Lajis N.H.; Tajuddin S.N.
• Format: Article
• Language: English
• Published: Taylor and Francis Ltd. 2015
• Online Access: https://www.scopus.com/inward/record.uri?eid=2-s2.0-84938959981&doi=10.1080%2f14786419.2014.985676&partnerID=40&md5=5f6250603f37b1e99488f7cf1966a24e

The stem bark extracts of Knema laurina inhibited the hydrogen peroxide (H2O2)-and aggregated amyloid β-peptide 1-42 length (Aβ1-42)-induced cell death in differentiated SH-SY5Y cells. Exposure of 250 μMH2O2 or 20 μM Aβ1-42 to the cells for 24 h reduced 50% of cell viability. Pretreatment of cells with ethyl acetate extract (EAE) or n-butanol extract (BE) at 300 μg/mL and then exposure to H2O2 protected the cells against the neurotoxic effects of H2O2. Besides, methanolic extract (ME) at 1 and 10 μg/mL exerted neuroprotective effect on Aβ1-42-induced toxicity to the cells. These results showed that EAE, BE and ME exhibited neuroprotective activities against H2O2-and Aβ1-42-induced cell death. Flavonoids (3-6) and β-sitosterol glucoside (8) were isolated from the EAE. Compound 1 was isolated from hexane extract, and compounds 2 and 7 were isolated from dichloromethane extract. All these observations provide the possible evidence for contribution in the neuroprotective effects. © 2014 Taylor and Francis.