| Summary: | The stem bark extracts of Knema laurina inhibited the hydrogen peroxide (H2O2)-and aggregated amyloid β-peptide 1-42 length (Aβ1-42)-induced cell death in differentiated SH-SY5Y cells. Exposure of 250 μMH2O2 or 20 μM Aβ1-42 to the cells for 24 h reduced 50% of cell viability. Pretreatment of cells with ethyl acetate extract (EAE) or n-butanol extract (BE) at 300 μg/mL and then exposure to H2O2 protected the cells against the neurotoxic effects of H2O2. Besides, methanolic extract (ME) at 1 and 10 μg/mL exerted neuroprotective effect on Aβ1-42-induced toxicity to the cells. These results showed that EAE, BE and ME exhibited neuroprotective activities against H2O2-and Aβ1-42-induced cell death. Flavonoids (3-6) and β-sitosterol glucoside (8) were isolated from the EAE. Compound 1 was isolated from hexane extract, and compounds 2 and 7 were isolated from dichloromethane extract. All these observations provide the possible evidence for contribution in the neuroprotective effects. © 2014 Taylor and Francis.
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