ACE2 activation by xanthenone prevents leptin-induced increases in blood pressure and proteinuria during pregnancy in Sprague-Dawley rats

• Published in: Reproductive Toxicology
• Main Author: Ibrahim H.S.; Froemming G.R.A.; Omar E.; Singh H.J.
• Format: Article
• Language: English
• Published: Elsevier Inc. 2014
• Online Access: https://www.scopus.com/inward/record.uri?eid=2-s2.0-84907553885&doi=10.1016%2fj.reprotox.2014.08.006&partnerID=40&md5=0b0a636c34dec04e5bcc0ef979d9fcd5

This study investigates the effect of ACE2 activation on leptin-induced changes in systolic blood pressure (SBP), proteinuria, endothelial activation and ACE2 expression during pregnancy in Sprague-Dawley rats. Pregnant rats were given subcutaneous injection of either saline, or leptin, or leptin plus xanthenone (ACE2 activator), or xanthenone (XTN) alone. SBP, serum ACE, ACE2, endothelin-1, E-selectin and ICAM-1 levels were estimated; also their gene expressions were determined in the kidney and aorta respectively. Compared to control, SBP was higher in the leptin-only treated group (P < 0.001) and lower in rats treated with xanthenone alone (P < 0.01). Proteinuria, markers of endothelial activation were significantly higher than controls in leptin-only treated rats (P < 0.05). ACE2 activity and expression were lower in leptin-only treated rats when compared to controls (P < 0.05). It seems, leptin administration during pregnancy significantly increases SBP, proteinuria, endothelial activation, but decreases ACE2 level and expression. These effects are prevented by concurrent administration of xanthenone. © 2014 Elsevier Inc.