Effects of the absorption behaviour of ZnO nanoparticles on cytotoxicity measurements

ZnO absorbs certain wavelengths of light and this behavior is more pronounced for nanoparticles of ZnO. As many toxicity measurements rely on measuring light transmission in cell lines, it is essential to determine how far this light absorption influences experimental toxicity measurements. The main...

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Published in:Journal of Nanomaterials
Main Author: Najim N.; Rusdi R.; Hamzah A.S.; Shaameri Z.; Mat Zain M.; Kamarulzaman N.
Format: Article
Language:English
Published: Hindawi Publishing Corporation 2014
Online Access:https://www.scopus.com/inward/record.uri?eid=2-s2.0-84896946910&doi=10.1155%2f2014%2f694737&partnerID=40&md5=d05831f17e7539bed5b6b7158e264a8c
id 2-s2.0-84896946910
spelling 2-s2.0-84896946910
Najim N.; Rusdi R.; Hamzah A.S.; Shaameri Z.; Mat Zain M.; Kamarulzaman N.
Effects of the absorption behaviour of ZnO nanoparticles on cytotoxicity measurements
2014
Journal of Nanomaterials
2014

10.1155/2014/694737
https://www.scopus.com/inward/record.uri?eid=2-s2.0-84896946910&doi=10.1155%2f2014%2f694737&partnerID=40&md5=d05831f17e7539bed5b6b7158e264a8c
ZnO absorbs certain wavelengths of light and this behavior is more pronounced for nanoparticles of ZnO. As many toxicity measurements rely on measuring light transmission in cell lines, it is essential to determine how far this light absorption influences experimental toxicity measurements. The main objective was to study the ZnO absorption and how this influenced the cytotoxicity measurements. The cytotoxicity of differently sized ZnO nanoparticles in normal and cancer cell lines derived from lung tissue (Hs888Lu), neuron-phenotypic cells (SH-SY5Y), neuroblastoma (SH-SY5Y), human histiocytic lymphoma (U937), and lung cancer (A549) was investigated. Our results demonstrate that the presence of ZnO affected the cytotoxicity measurements due to the absorption characteristic of ZnO nanoparticles. The data revealed that the ZnO nanoparticles with an average particle size of around 85.7 nm and 190 nm showed cytotoxicity towards U937, SH-SY5Y, differentiated SH-SY5Y, and Hs888Lu cell lines. No effect on the A549 cells was observed. It was also found that the cytotoxicity of ZnO was particle size, concentration, and time dependent. These studies are the first to quantify the influence of ZnO nanoparticles on cytotoxicity assays. Corrections for absorption effects were carried out which gave an accurate estimation of the concentrations that produce the cytotoxic effects. © 2014 Nigar Najim et al.
Hindawi Publishing Corporation
16874110
English
Article
All Open Access; Hybrid Gold Open Access
author Najim N.; Rusdi R.; Hamzah A.S.; Shaameri Z.; Mat Zain M.; Kamarulzaman N.
spellingShingle Najim N.; Rusdi R.; Hamzah A.S.; Shaameri Z.; Mat Zain M.; Kamarulzaman N.
Effects of the absorption behaviour of ZnO nanoparticles on cytotoxicity measurements
author_facet Najim N.; Rusdi R.; Hamzah A.S.; Shaameri Z.; Mat Zain M.; Kamarulzaman N.
author_sort Najim N.; Rusdi R.; Hamzah A.S.; Shaameri Z.; Mat Zain M.; Kamarulzaman N.
title Effects of the absorption behaviour of ZnO nanoparticles on cytotoxicity measurements
title_short Effects of the absorption behaviour of ZnO nanoparticles on cytotoxicity measurements
title_full Effects of the absorption behaviour of ZnO nanoparticles on cytotoxicity measurements
title_fullStr Effects of the absorption behaviour of ZnO nanoparticles on cytotoxicity measurements
title_full_unstemmed Effects of the absorption behaviour of ZnO nanoparticles on cytotoxicity measurements
title_sort Effects of the absorption behaviour of ZnO nanoparticles on cytotoxicity measurements
publishDate 2014
container_title Journal of Nanomaterials
container_volume 2014
container_issue
doi_str_mv 10.1155/2014/694737
url https://www.scopus.com/inward/record.uri?eid=2-s2.0-84896946910&doi=10.1155%2f2014%2f694737&partnerID=40&md5=d05831f17e7539bed5b6b7158e264a8c
description ZnO absorbs certain wavelengths of light and this behavior is more pronounced for nanoparticles of ZnO. As many toxicity measurements rely on measuring light transmission in cell lines, it is essential to determine how far this light absorption influences experimental toxicity measurements. The main objective was to study the ZnO absorption and how this influenced the cytotoxicity measurements. The cytotoxicity of differently sized ZnO nanoparticles in normal and cancer cell lines derived from lung tissue (Hs888Lu), neuron-phenotypic cells (SH-SY5Y), neuroblastoma (SH-SY5Y), human histiocytic lymphoma (U937), and lung cancer (A549) was investigated. Our results demonstrate that the presence of ZnO affected the cytotoxicity measurements due to the absorption characteristic of ZnO nanoparticles. The data revealed that the ZnO nanoparticles with an average particle size of around 85.7 nm and 190 nm showed cytotoxicity towards U937, SH-SY5Y, differentiated SH-SY5Y, and Hs888Lu cell lines. No effect on the A549 cells was observed. It was also found that the cytotoxicity of ZnO was particle size, concentration, and time dependent. These studies are the first to quantify the influence of ZnO nanoparticles on cytotoxicity assays. Corrections for absorption effects were carried out which gave an accurate estimation of the concentrations that produce the cytotoxic effects. © 2014 Nigar Najim et al.
publisher Hindawi Publishing Corporation
issn 16874110
language English
format Article
accesstype All Open Access; Hybrid Gold Open Access
record_format scopus
collection Scopus
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