Antibiofilm properties of chemically synthesized silver nanoparticles found against Pseudomonas aeruginosa

Nanomedicine is now being introduced as a recent trend in the field of medicine. It has been documented that metal nanoparticles have antimicrobial effects for bacteria, fungi and viruses. Recent advances in technology has revived the use of silver nanoparticles in the medical field; treatment, diag...

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Published in:Journal of Nanobiotechnology
Main Author: Palanisamy N.K.; Ferina N.; Amirulhusni A.N.; Mohd-Zain Z.; Hussaini J.; Ping L.J.; Durairaj R.
Format: Article
Language:English
Published: 2014
Online Access:https://www.scopus.com/inward/record.uri?eid=2-s2.0-84892394603&doi=10.1186%2f1477-3155-12-2&partnerID=40&md5=d079da8db7701e4683e183a39ab37873
id 2-s2.0-84892394603
spelling 2-s2.0-84892394603
Palanisamy N.K.; Ferina N.; Amirulhusni A.N.; Mohd-Zain Z.; Hussaini J.; Ping L.J.; Durairaj R.
Antibiofilm properties of chemically synthesized silver nanoparticles found against Pseudomonas aeruginosa
2014
Journal of Nanobiotechnology
12
1
10.1186/1477-3155-12-2
https://www.scopus.com/inward/record.uri?eid=2-s2.0-84892394603&doi=10.1186%2f1477-3155-12-2&partnerID=40&md5=d079da8db7701e4683e183a39ab37873
Nanomedicine is now being introduced as a recent trend in the field of medicine. It has been documented that metal nanoparticles have antimicrobial effects for bacteria, fungi and viruses. Recent advances in technology has revived the use of silver nanoparticles in the medical field; treatment, diagnosis, monitoring and control of disease. It has been used since ancient times for treating wide range of illnesses. Bacterial cells adheres to surfaces and develop structures known as biofilms. These structures are natural survival strategy of the bacteria to invade the host. They are more tolerant to commonly used antimicrobial agents, thus being more difficult to be controlled. This leads to increase in severity of infection. In this study, we have investigated the effect of silver nanoparticles in the formation of biofilm in multidrug resistant strains of Pseudomonas aeruginosa. Observation showed that biofilm formation occurred at bacterial concentration of 106 cfu/ml for the sensitive strain of P. aeruginosa while in the resistant strain, the biofilm was evident at bacterial concentration of about 103 cfu/ml. The biofilm were then tested against various concentrations of silver nanoparticles to determine the inhibitory effect of the silver nanoparticles. In the sensitive strain, 20 μg/ml of silver nanoparticles inhibited the growth optimally at bacterial concentration of 104 cfu/ml with an inhibition rate of 67%. Similarly, silver nanoparticles inhibited the formation of biofilm in the resistant strain at an optimal bacterial concentration of 105 cfu/ml with an inhibition rate of 56%. Thus, silver nanoparticles could be used as a potential alternative therapy to reduce severity of disease due to P. aeruginosa infections. © 2014 Palanisamy et al.; licensee BioMed Central Ltd.

14773155
English
Article
All Open Access; Gold Open Access
author Palanisamy N.K.; Ferina N.; Amirulhusni A.N.; Mohd-Zain Z.; Hussaini J.; Ping L.J.; Durairaj R.
spellingShingle Palanisamy N.K.; Ferina N.; Amirulhusni A.N.; Mohd-Zain Z.; Hussaini J.; Ping L.J.; Durairaj R.
Antibiofilm properties of chemically synthesized silver nanoparticles found against Pseudomonas aeruginosa
author_facet Palanisamy N.K.; Ferina N.; Amirulhusni A.N.; Mohd-Zain Z.; Hussaini J.; Ping L.J.; Durairaj R.
author_sort Palanisamy N.K.; Ferina N.; Amirulhusni A.N.; Mohd-Zain Z.; Hussaini J.; Ping L.J.; Durairaj R.
title Antibiofilm properties of chemically synthesized silver nanoparticles found against Pseudomonas aeruginosa
title_short Antibiofilm properties of chemically synthesized silver nanoparticles found against Pseudomonas aeruginosa
title_full Antibiofilm properties of chemically synthesized silver nanoparticles found against Pseudomonas aeruginosa
title_fullStr Antibiofilm properties of chemically synthesized silver nanoparticles found against Pseudomonas aeruginosa
title_full_unstemmed Antibiofilm properties of chemically synthesized silver nanoparticles found against Pseudomonas aeruginosa
title_sort Antibiofilm properties of chemically synthesized silver nanoparticles found against Pseudomonas aeruginosa
publishDate 2014
container_title Journal of Nanobiotechnology
container_volume 12
container_issue 1
doi_str_mv 10.1186/1477-3155-12-2
url https://www.scopus.com/inward/record.uri?eid=2-s2.0-84892394603&doi=10.1186%2f1477-3155-12-2&partnerID=40&md5=d079da8db7701e4683e183a39ab37873
description Nanomedicine is now being introduced as a recent trend in the field of medicine. It has been documented that metal nanoparticles have antimicrobial effects for bacteria, fungi and viruses. Recent advances in technology has revived the use of silver nanoparticles in the medical field; treatment, diagnosis, monitoring and control of disease. It has been used since ancient times for treating wide range of illnesses. Bacterial cells adheres to surfaces and develop structures known as biofilms. These structures are natural survival strategy of the bacteria to invade the host. They are more tolerant to commonly used antimicrobial agents, thus being more difficult to be controlled. This leads to increase in severity of infection. In this study, we have investigated the effect of silver nanoparticles in the formation of biofilm in multidrug resistant strains of Pseudomonas aeruginosa. Observation showed that biofilm formation occurred at bacterial concentration of 106 cfu/ml for the sensitive strain of P. aeruginosa while in the resistant strain, the biofilm was evident at bacterial concentration of about 103 cfu/ml. The biofilm were then tested against various concentrations of silver nanoparticles to determine the inhibitory effect of the silver nanoparticles. In the sensitive strain, 20 μg/ml of silver nanoparticles inhibited the growth optimally at bacterial concentration of 104 cfu/ml with an inhibition rate of 67%. Similarly, silver nanoparticles inhibited the formation of biofilm in the resistant strain at an optimal bacterial concentration of 105 cfu/ml with an inhibition rate of 56%. Thus, silver nanoparticles could be used as a potential alternative therapy to reduce severity of disease due to P. aeruginosa infections. © 2014 Palanisamy et al.; licensee BioMed Central Ltd.
publisher
issn 14773155
language English
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accesstype All Open Access; Gold Open Access
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