Prevalence of verotoxin gene among the clinical isolates of enteropathogenic Escherichia coli in Malaysia

Prevalence of verotoxin gene among the clinical isolates of enteropathogenic Escherichia coli in Malaysia

A total of thirty two enteropathogenic Escherichia coli (EPEC) isolated from Miri hospital, Malaysia, from children with diarrhea were examined. Polymerase chain reaction used in this study to detect verotoxin (VT) gene proved to be rapid, sensitive, specific and reproducible. Two sets of primers (V...

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Published in:World Applied Sciences Journal
Main Author: Nazmul M.H.M.; Rashid M.A.; Jamal H.
Format: Article
Language:English
Published: 2014
Online Access:https://www.scopus.com/inward/record.uri?eid=2-s2.0-84892377216&doi=10.5829%2fidosi.wasj.2014.29.02.1211&partnerID=40&md5=55ee4758bf16da88983e5e963cfa5fef
id 2-s2.0-84892377216
spelling 2-s2.0-84892377216
Nazmul M.H.M.; Rashid M.A.; Jamal H.
Prevalence of verotoxin gene among the clinical isolates of enteropathogenic Escherichia coli in Malaysia
2014
World Applied Sciences Journal
29
2
10.5829/idosi.wasj.2014.29.02.1211
https://www.scopus.com/inward/record.uri?eid=2-s2.0-84892377216&doi=10.5829%2fidosi.wasj.2014.29.02.1211&partnerID=40&md5=55ee4758bf16da88983e5e963cfa5fef
A total of thirty two enteropathogenic Escherichia coli (EPEC) isolated from Miri hospital, Malaysia, from children with diarrhea were examined. Polymerase chain reaction used in this study to detect verotoxin (VT) gene proved to be rapid, sensitive, specific and reproducible. Two sets of primers (VT1 and VT2) were used. Both the primers produced a fragment of 348 bp of VT1 gene and 584 bp of VT2 gene, respectively. It was found that 20 E. coli isolates (40%) carried VT1 gene whereas no isolate carried VT2 gene.VT1 was found to be the most common verotoxin among the EPEC strains isolated from clinical sources in Malaysia. © IDOSI Publications, 2014.

19916426
English
Article
author Nazmul M.H.M.; Rashid M.A.; Jamal H.
spellingShingle Nazmul M.H.M.; Rashid M.A.; Jamal H.
Prevalence of verotoxin gene among the clinical isolates of enteropathogenic Escherichia coli in Malaysia
author_facet Nazmul M.H.M.; Rashid M.A.; Jamal H.
author_sort Nazmul M.H.M.; Rashid M.A.; Jamal H.
title Prevalence of verotoxin gene among the clinical isolates of enteropathogenic Escherichia coli in Malaysia
title_short Prevalence of verotoxin gene among the clinical isolates of enteropathogenic Escherichia coli in Malaysia
title_full Prevalence of verotoxin gene among the clinical isolates of enteropathogenic Escherichia coli in Malaysia
title_fullStr Prevalence of verotoxin gene among the clinical isolates of enteropathogenic Escherichia coli in Malaysia
title_full_unstemmed Prevalence of verotoxin gene among the clinical isolates of enteropathogenic Escherichia coli in Malaysia
title_sort Prevalence of verotoxin gene among the clinical isolates of enteropathogenic Escherichia coli in Malaysia
publishDate 2014
container_title World Applied Sciences Journal
container_volume 29
container_issue 2
doi_str_mv 10.5829/idosi.wasj.2014.29.02.1211
url https://www.scopus.com/inward/record.uri?eid=2-s2.0-84892377216&doi=10.5829%2fidosi.wasj.2014.29.02.1211&partnerID=40&md5=55ee4758bf16da88983e5e963cfa5fef
description A total of thirty two enteropathogenic Escherichia coli (EPEC) isolated from Miri hospital, Malaysia, from children with diarrhea were examined. Polymerase chain reaction used in this study to detect verotoxin (VT) gene proved to be rapid, sensitive, specific and reproducible. Two sets of primers (VT1 and VT2) were used. Both the primers produced a fragment of 348 bp of VT1 gene and 584 bp of VT2 gene, respectively. It was found that 20 E. coli isolates (40%) carried VT1 gene whereas no isolate carried VT2 gene.VT1 was found to be the most common verotoxin among the EPEC strains isolated from clinical sources in Malaysia. © IDOSI Publications, 2014.
publisher
issn 19916426
language English
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