Genetic relationship between roselle (Hibiscus sabdariffa L.) and kenaf (Hibiscus cannabinus L.) accessions through optimization of PCR based RAPD method

The PCR based RAPD analysis was used to study genetic relationship among roselle and kenaf accessions. Extraction of genomic DNA samples of nine roselle (Hibiscus sabdariffa L.) and seven kenaf (Hibiscus cannabinus L.) accessions were successfully performed by Cetyl Trimethyl Ammonium Bromide (CTAB)...

Full description

Bibliographic Details
Published in:Emirates Journal of Food and Agriculture
Main Author: Omalsaad; Islam A.K.M.A.; Jahan M.A.; Yaakob Z.; Osman M.
Format: Article
Language:English
Published: United Arab Emirates University 2014
Online Access:https://www.scopus.com/inward/record.uri?eid=2-s2.0-84891337268&doi=10.9755%2fejfa.v26i3.16498&partnerID=40&md5=2e4055d143d5d5fe3349d32732eaf026
id 2-s2.0-84891337268
spelling 2-s2.0-84891337268
Omalsaad; Islam A.K.M.A.; Jahan M.A.; Yaakob Z.; Osman M.
Genetic relationship between roselle (Hibiscus sabdariffa L.) and kenaf (Hibiscus cannabinus L.) accessions through optimization of PCR based RAPD method
2014
Emirates Journal of Food and Agriculture
26
3
10.9755/ejfa.v26i3.16498
https://www.scopus.com/inward/record.uri?eid=2-s2.0-84891337268&doi=10.9755%2fejfa.v26i3.16498&partnerID=40&md5=2e4055d143d5d5fe3349d32732eaf026
The PCR based RAPD analysis was used to study genetic relationship among roselle and kenaf accessions. Extraction of genomic DNA samples of nine roselle (Hibiscus sabdariffa L.) and seven kenaf (Hibiscus cannabinus L.) accessions were successfully performed by Cetyl Trimethyl Ammonium Bromide (CTAB) method. RAPD analysis using two chloroplast primers and one mitochondrial primer produced about 62 bands of different sizes. Most of the fragments were polymorphic where 2-3 fragments were monomorphic in each primer. Sixteen accessions were classified into two main groups A and B through dendogram generated using RAPD polymorphism. Five kenaf accessions were included in group A, whereas nine roselle accessions and two kenaf accessions were included in group B. A group was formed with a range of similarity coefficients of 0.5333 to 0.8780 having all roselle accessions. Bengkalis was different from Hibiscus sp but it was close to roselle accessions. Noonsoon was very close to roselle accessions having a range of jaccard similarity coefficients of 0.5000 to 0.6326. It was evident from jaccad similarity coefficient of 0.8780 that the roselle varieties UKMR 1 and UKMR 2 were genetically closer to each other. On the other hand, V36 and Thainung showed the highest similarity coefficient (0.7878) among kenaf accessions in group A. This study has successfully differentiated between two species by using chloroplast and mitochondrial markers which was due to high level of polymorphism. However, the chloroplast primers were more effective than M13 to study the genetic variation between roselle and kenaf.
United Arab Emirates University
2079052X
English
Article
All Open Access; Gold Open Access
author Omalsaad; Islam A.K.M.A.; Jahan M.A.; Yaakob Z.; Osman M.
spellingShingle Omalsaad; Islam A.K.M.A.; Jahan M.A.; Yaakob Z.; Osman M.
Genetic relationship between roselle (Hibiscus sabdariffa L.) and kenaf (Hibiscus cannabinus L.) accessions through optimization of PCR based RAPD method
author_facet Omalsaad; Islam A.K.M.A.; Jahan M.A.; Yaakob Z.; Osman M.
author_sort Omalsaad; Islam A.K.M.A.; Jahan M.A.; Yaakob Z.; Osman M.
title Genetic relationship between roselle (Hibiscus sabdariffa L.) and kenaf (Hibiscus cannabinus L.) accessions through optimization of PCR based RAPD method
title_short Genetic relationship between roselle (Hibiscus sabdariffa L.) and kenaf (Hibiscus cannabinus L.) accessions through optimization of PCR based RAPD method
title_full Genetic relationship between roselle (Hibiscus sabdariffa L.) and kenaf (Hibiscus cannabinus L.) accessions through optimization of PCR based RAPD method
title_fullStr Genetic relationship between roselle (Hibiscus sabdariffa L.) and kenaf (Hibiscus cannabinus L.) accessions through optimization of PCR based RAPD method
title_full_unstemmed Genetic relationship between roselle (Hibiscus sabdariffa L.) and kenaf (Hibiscus cannabinus L.) accessions through optimization of PCR based RAPD method
title_sort Genetic relationship between roselle (Hibiscus sabdariffa L.) and kenaf (Hibiscus cannabinus L.) accessions through optimization of PCR based RAPD method
publishDate 2014
container_title Emirates Journal of Food and Agriculture
container_volume 26
container_issue 3
doi_str_mv 10.9755/ejfa.v26i3.16498
url https://www.scopus.com/inward/record.uri?eid=2-s2.0-84891337268&doi=10.9755%2fejfa.v26i3.16498&partnerID=40&md5=2e4055d143d5d5fe3349d32732eaf026
description The PCR based RAPD analysis was used to study genetic relationship among roselle and kenaf accessions. Extraction of genomic DNA samples of nine roselle (Hibiscus sabdariffa L.) and seven kenaf (Hibiscus cannabinus L.) accessions were successfully performed by Cetyl Trimethyl Ammonium Bromide (CTAB) method. RAPD analysis using two chloroplast primers and one mitochondrial primer produced about 62 bands of different sizes. Most of the fragments were polymorphic where 2-3 fragments were monomorphic in each primer. Sixteen accessions were classified into two main groups A and B through dendogram generated using RAPD polymorphism. Five kenaf accessions were included in group A, whereas nine roselle accessions and two kenaf accessions were included in group B. A group was formed with a range of similarity coefficients of 0.5333 to 0.8780 having all roselle accessions. Bengkalis was different from Hibiscus sp but it was close to roselle accessions. Noonsoon was very close to roselle accessions having a range of jaccard similarity coefficients of 0.5000 to 0.6326. It was evident from jaccad similarity coefficient of 0.8780 that the roselle varieties UKMR 1 and UKMR 2 were genetically closer to each other. On the other hand, V36 and Thainung showed the highest similarity coefficient (0.7878) among kenaf accessions in group A. This study has successfully differentiated between two species by using chloroplast and mitochondrial markers which was due to high level of polymorphism. However, the chloroplast primers were more effective than M13 to study the genetic variation between roselle and kenaf.
publisher United Arab Emirates University
issn 2079052X
language English
format Article
accesstype All Open Access; Gold Open Access
record_format scopus
collection Scopus
_version_ 1809678161998249984