An alternate method for DNA and RNA extraction from clotted blood

QR Code

An alternate method for DNA and RNA extraction from clotted blood

We developed an alternative method to extract DNA and RNA from clotted blood for genomic and molecular investigations. A combination of the TRIzol method and the QIAamp spin column were used to extract RNA from frozen clotted blood. Clotted blood was sonicated and then the QIAamp DNA Blood Mini Kit...

Full description

Bibliographic Details
Published in:	Genetics and Molecular Research
Main Author:	Zakaria Z.; Umi S.H.; Mokhtar S.S.; Mokhtar U.; Zaiharina M.Z.; Aziz A.T.A.; Hoh B.P.
Format:	Article
Language:	English
Published:	Fundacao de Pesquisas Cientificas de Ribeirao Preto 2013
Online Access:	https://www.scopus.com/inward/record.uri?eid=2-s2.0-84887342624&doi=10.4238%2f2013.February.4.4&partnerID=40&md5=9133d2e4ef54b6b1cbe8c87981147dd4

Similar Items

Effect of DNase treatment on RNA extraction from preimplantation murine embryos
by: Norhazlin J.; Nor-Ashikin M.N.K.; Hoh B.P.; Kadir S.H.S.A.; Norita S.; Mohd-Fazirul M.; Wan-Hafizah W.J.; Razif D.; Rajikin M.H.; Abdullah B.
Published: (2015)

Common variants of chemokine receptor gene CXCR3 and its ligands CXCL10 and CXCL11 associated with vascular permeability of dengue infection in peninsular Malaysia
by: Hoh B.P.; Umi-Shakina H.; Zuraihan Z.; Zaiharina M.Z.; Rafidah-Hanim S.; Mahiran M.; Nik Khairudin N.Y.; Benedict L.H.S.; Masliza Z.; Christopher K.C.L.; Sazaly A.B.
Published: (2015)

A novel rare copy number variant of the ABCF1 gene identified among dengue fever patients from Peninsular Malaysia
by: Hoh B.P.; Sam S.S.; Umi S.H.; Mahiran M.; Nik Khairudin N.Y.; Rafidah Hanim S.; AbuBakar S.
Published: (2014)

Comparison of PLS Discriminant Analysis and supervised SOMs for Blood Brain Barrier activity
by: Jaafar M.Z.; Mokhtar M.; Hasan M.N.; Bakhari N.A.; Brereton R.G.
Published: (2015)

Nodamura virus B2 amino terminal domain sensitivity to small interfering RNA
by: Shaik Syed Ali P.; John J.; Selvaraj M.; Kek T.L.; Salleh M.Z.
Published: (2015)

The Capability of Rainwater Harvesting as an Alternative Water Supply
by: Tholibon D.A.; Mokhtar H.; Latib F.W.M.; Ismail M.I.; Nujid M.M.; Salam R.
Published: (2024)

Classification of Breast Cancer Subtypes using Microarray RNA Expression Data
by: Suhiman M.S.; Deni S.M.; Japeri A.Z.U.-S.M.; Asmat A.; Wang L.
Published: (2025)

Assessing awareness and acceptance of storing DNA profile in forensic DNA databank Malaysia
by: Nawani S.B.M.; Khamis K.A.; Zakaria Z.
Published: (2023)

UNVEILING THE SIGNIFICANCE OF PROPOSING HIBAH AS AN ALTERNATIVE TO NAFKAH SECURITY FOR WIDOWS
by: Azmi A.F.; Ahmad N.L.; Zakaria M.Z.; Saleh N.S.S.N.; Hasbullah M.
Published: (2024)

Observational study of the status of coronary risk biomarkers among Negritos with metabolic syndrome in the east coast of Malaysia
by: Mokhsin A.; Mokhtar S.S.; Mohd Ismail A.; M Nor F.; Shaari S.A.; Nawawi H.; Yusoff K.; Abdul Rahman T.; Hoh B.P.
Published: (2018)

Blood glucose lowering property of water in oral insulin-fed diabetic rats
by: Wong T.W.; Sumiran N.; Mohd Mokhtar M.T.; Kadir A.
Published: (2012)

Species Identification of Potential Probiotic Lactic Acid Bacteria Isolated from Malaysian Fermented Food Based on 16S Ribosomal RNA (16S rRNA) and Internal Transcribed Spacer (ITS) Sequences
by: Ilyanie Y.; Huda Faujan N.; Ida Muryany M.Y.
Published: (2023)

Thermoresponsive curcumin/DsiRNA nanoparticle gels for the treatment of diabetic wounds: Synthesis and drug release
by: Katas H.; Wen C.Y.; Siddique M.I.; Hussain Z.; Mohd Fadhil F.H.
Published: (2017)

DNA Extraction from Bloodstained Fabric Samples Immersed in Different Types of Water
by: Rajagopal K.; Sabri A.S.A.; Md Yusoff N.A.B.
Published: (2023)

Ethanol Extract of Centella asiatica Improved Methamphetamine-Induced Neurotoxicity on Mouse Model via Stimulating Superoxide Dismutase II and microRNA-34A Expression
by: Shamsuddin N.; Zain M.M.; Adenan M.I.; Noorden M.S.A.
Published: (2023)

Natural selection and local adaptation of blood pressure regulation and their perspectives on precision medicine in hypertension
by: Hoh B.-P.; Abdul Rahman T.; Yusoff K.
Published: (2019)

MicroRNA Methylome Signature and Their Functional Roles in Colorectal Cancer Diagnosis, Prognosis, and Chemoresistance
by: Baharudin R.; Rus Bakarurraini N.Q.; Ismail I.; Lee L.-H.; Ab Mutalib N.S.
Published: (2022)

CLASSIFICATION OF BLOOD SUBSTITUTES
by: Shamshul B.S.S.; Mohd N.S.N.F.; Noordin S.S.; Mohd N.M.N.; Zabidi M.A.
Published: (2023)

Alternative methods for forecasting variations in hospital bed admission
by: Shariff S.S.R.; Suhaimi M.A.; Zahari S.M.; Derasit Z.
Published: (2018)

An improved rapid genotyping method for the study of beta-2 adrenergic receptor gene polymorphisms using single tube allele specific multiplex PCR
by: Zilfalil B.A.; Hoh B.P.; Nizam M.Z.; Liza-Sharmini A.T.; Teh L.K.; Ismail R.
Published: (2006)

Evaluation of the world health organization's 1997 and 2009 dengue classifications in hospitalized dengue patients in Malaysia
by: Zakaria Z.; Zainordin N.A.; Sim B.L.H.; Zaid M.; Haridan U.S.; Aziz A.T.A.; Shueb R.H.; Mustafa M.; Yusoff N.K.N.; Malik A.S.; Lee C.K.C.; Abubakar S.; Hoh B.P.
Published: (2014)

Segregation and genetic linkage analyses of river catfish, Mystus nemurus, based on microsatellite markers
by: Hoh B.P.; Siraj S.S.; Tan S.G.; Yusoff K.
Published: (2013)

Prevalence and risk factors of hepatitis C virus infection in haemodialysis patients: Testing antibodies, RNA and genotypes
by: Al-Kubaisy W.; Noor N.A.M.; Ibrahim N.S.N.; Al-Nasirie U.
Published: (2015)

Targeting genetic tool for long non-coding RNA of cancer stem cells with aptamer-guided nanocarriers
by: Zaiki Y.; Wong T.W.
Published: (2021)

A genome-wide characterization of copy number variations in native populations of Peninsular Malaysia
by: Fu R.; Mokhtar S.S.; Phipps M.E.; Hoh B.-P.; Xu S.
Published: (2018)

Unraveling the bacterial diversity of cangar hot spring, indonesia by next generation sequencing of 16s rRNA gene
by: Geraldi A.; Tay C.C.; Ni'matuzahroh; Fatimah; Hanafi W.N.W.
Published: (2021)

Atomisation of nanometre-scaled jasmine flower extracts using electrospray method
by: Rahmam S.; Naim M.N.; Bakar N.F.A.; Mokhtar M.N.
Published: (2022)

Effects of Standardized Aqueous Ethanolic Extract of Leaves of Ficus Deltoidea var. Trengganuensis on Blood Pressure in Spontaneously Hypertensive Rats
by: Radjeni Z.; Aziz M.A.; Azis N.A.; Kamal M.S.A.; Singh H.J.
Published: (2024)

Storage effect of air liur sample on quality of genomic DNA; [Kesan Penyimpanan Sampel Air Liur Terhadap Kualiti DNA Genom]
by: Wahab R.M.A.; Nasri F.A.M.; Abidin I.Z.Z.; Ariffin Z.Z.; Yazid M.D.; Ariffin S.H.Z.
Published: (2017)

Mechanisms of gastroprotection of methanol extract of Melastoma malabathricum leaves
by: Zakaria Z.A.; Balan T.; Mamat S.S.; Mohtarrudin N.; Kek T.L.; Salleh M.Z.
Published: (2015)

Molecular characterization of microbial community diversity associated with blood cockles (Anadara granosa) in blood cockle farms
by: Zarkasi K.Z.; Shukri A.A.A.; Nazari T.F.; Abdullah A.A.A.; Daud F.
Published: (2019)

Neurodevelopmental effects of prenatal Bisphenol A exposure on the role of microRNA regulating NMDA receptor subunits in the male rat hippocampus
by: Nayan N.M.; Kadir S.H.S.A.; Husin A.; Siran R.
Published: (2024)

Neurodevelopmental effects of prenatal Bisphenol A exposure on the role of microRNA regulating NMDA receptor subunits in the male rat hippocampus
by: Nayan, et al.
Published: (2024)

A Review of the Emulsification Method for Alternative Fuels Used in Diesel Engines
by: Hamid M.F.; Teoh Y.H.; Idroas M.Y.; Mohamed M.; Sa’ad S.; Mat S.C.; Abdullah M.K.; Le T.D.; How H.G.; Nguyen H.T.
Published: (2022)

Simulation of Flow Profile Response to Alternate Bar Formation in Rivers
by: Salleh M.Z.M.; Ibrahim Z.; Jumain M.; Saari R.; Fathullah M.F.M.; Ismail Z.; Jamal M.H.
Published: (2020)

How to help patients to control their blood pressure? Blood pressure control and its predictor
by: Hassan Y.; Aziz N.A.; Kassab Y.W.; Elgasim I.; Shaharuddin S.; Al-Worafi Y.M.A.; Zulkifly H.H.; Ming L.C.
Published: (2014)

RAPID IDENTIFICATION AND CHARACTERIZATION OF THE Lactobacillus STRAINS BY USING 16S-23S rRNA GENE INTERGENIC SPACER REGION AND recA GENES
by: Ida Muryany M.Y.; Chin Y.K.; Ina-Salwany M.Y.
Published: (2023)

Cyclic voltammetry readout circuitry for dna biosensor application
by: Hamzah I.H.; Malik A.A.; Rahim A.F.A.; Soh Z.H.C.
Published: (2021)

Performance of concrete with mining sand as an alternative non-conventional material
by: Harith Z.Y.H.; Shavarebi K.A.
Published: (2005)

Matching Game Genre with Lesson Content – A Development of Blood Circulation Racing Game
by: Ahmadon F.; Selahuddeen M.Z.; Rahim E.E.A.; Rusli H.M.
Published: (2024)

Cannot write session to /tmp/vufind_sessions/sess_dniubqa3gt8lvjmga91qegjl9g