Total phenolic content, total flavonoid content, antioxidant and antimicrobial activities of Malaysian Shorea

• Published in: Research Journal of Medicinal Plant
• Main Author: Ahmat N.; Norizan N.; Mohamad S.A.S.; Nazri N.A.A.M.; Ramli S.S.A.; Kasim S.N.M.; Zain W.Z.W.M.
• Format: Article
• Language: English
• Published: 2012
• Online Access: https://www.scopus.com/inward/record.uri?eid=2-s2.0-84865226390&doi=10.3923%2frjmp.2012.489.499&partnerID=40&md5=fff5db633e50ed79d9c8c25f1bc59d17

Five Shorea species which are endemic plants of Malaysia were used in this study. The methanol extract of Shorea acuminata, Shorea macroptera, Shorea leprosula, Shorea bracteolata and Shorea resinosa were investigated for total phenolic contents (TPC), total flavonoid contents, radical scavenging properties (DPPH), in vitro antioxidant and antimicrobial activities. For the antimicrobial activity, the extracts were tested against four bacteria; Gram negative bacteria Escherichia coli, Gram positive bacteria Staphylococcus pyogenes, Staphylococcus aureus and Bacillus subtilis and two fungi, Candida albicans and Aspergillus niger. The result of TPC analysis showed that S. acuminata displayed highest total phenolic content, 2731±0.09 mg/100 g, while in total flavonoid content, S. resinosa displayed the highest amount of flavonoid, 956.73±0.01 mg/100 g. However, in DPPH analysis, the samples of S. resinosa, S. macroptera and S. acuminate displayed better scavenging activity as compared to butylatedhydroxytoluene (BHT). The in vitro antioxidant activity indicated that S. macroptera showed the highest percent inhibition both in ferric thiocyanate method (FTC) (98.68%) and thiobarbituric acid method (TBA) analysis. In antimicrobial activity, S. resinosa displayed moderate inhibition against Gram negative bacteria Escherichia coli, Gram positive bacteria Streptococcus pyogenes, Staphylococcus aureus and Bacillus subtilis. Other plants were found to display selective inhibition against microbes. © AULA Academic Journals inc.