Addition of low-cost additives improves real time PCR assay of GC rich FMR1 region utilizing taqman probe
Objective: To obtain specific amplification, demonstrating that these low-cost additives can improve the amplification of the GC rich region in FMR1 gene, not only in PCR, but Real-time PCR as well. Methods: The basic reaction for the real time PCR were set up in a total volume of 20 ··l containing...
| Published in: | International Medical Journal |
|---|---|
| Main Author: | |
| Format: | Article |
| Language: | English |
| Published: |
2011
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| Online Access: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-83655165022&partnerID=40&md5=7b640f527d6f4a8eeabaf02885e0efaa |