Isolation and cloning of an inulinase gene from an endophytic bacteria

• Published in: Advances in Environmental Biology
• Main Author: Basran N.F.; Mustafa S.; Shamsuddin R.A.; Ali A.M.; Noormi R.; Subramaniam S.
• Format: Article
• Language: English
• Published: 2010
• Online Access: https://www.scopus.com/inward/record.uri?eid=2-s2.0-79960369222&partnerID=40&md5=a9fe378563ec9935e86030c8e9dc819c

In this study, an endophytic bacterium was isolated from Black Solenostemon scutellarioides plant and was named as A5. A simple and rapid method is described for the detection of inulinase-producing bacterial endophyte on agar plate using Remazol Brilliant Blue-inulin as substrate. The inulinase activity of A5 bacteria isolate was detected by clear zone surroundings the colonies. The inulinase gene obtained from A5 bacterium was amplified by the polymerase chain reaction. Degenerate primers were used specifically designed to pick up the inulinase gene. pGEM-T Easy and E. coli JM109 were used as cloning vector and host strain, respectively. The results showed that the partial sequence of inulinase gene consisted of 449bp. From the comparison with partial amino acid sequence of inulinase, the deduced polypeptide showed high sequence similarity to Paenibaccillus polymyxa and Geobacillus stearothermophilus exo-inulinases.