A validated LC method for the quantitation of cefotaxime in ph-sensitive nanoparticles

• Published in: Chromatographia
• Main Author: Pereira R.; Julianto T.; Ang P.-K.; Ling S.S.-N.; Barbosa C.M.; Yuen K.-H.; Majeed A.B.A.
• Format: Article
• Language: English
• Published: 2010
• Online Access: https://www.scopus.com/inward/record.uri?eid=2-s2.0-77952095820&doi=10.1365%2fs10337-010-1481-5&partnerID=40&md5=675f8eaaaa6680fc089418f79c369a10

A sensitive and rapid routine LC method was validated for measuring cefotaxime incorporated in three different pH-sensitive nanoparticles. The drug was chromatographed on a C18 reversed-phase column; the mobile phase used was 0.05 M aqueous ammonium acetate, acetonitrile and tetrahydrofuran (87:11:2, v/v) adjusted to pH 5.5 with acetic acid. The flow rate was 1 mL min-1 and cefotaxime was quantified at 254 nm, with a sensitivity range of 0.005 AUFS. The validated method was specific, linear (R 2 ≥ 0.999), precise and accurate in a concentration range of 0.2-50.0 μg mL-1. The method was rapid, selective and suitable for evaluation of cefotaxime in pH-sensitive Eudragit nanoparticles. © 2010 Vieweg+Teubner Verlag | Springer Fachmedien Wiesbaden GmbH.