Factors affecting the accumulation of 9-methoxycanthin-6-one in callus cultures of Eurycoma longifolia
A study was conducted to improve 9-methoxycanthin-6-one productivity (potential anti-tumour compound) from callus cultures of Eurycoma longifolia (Tongkat Ali). Several factors affecting 9-methoxycanthin-6-one production in callus cultures such as different medium compositions and physical factors w...
Published in: | Journal of Forestry Research |
---|---|
Main Author: | |
Format: | Article |
Language: | English |
Published: |
Springer Nature
2009
|
Online Access: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-58449128324&doi=10.1007%2fs11676-009-0010-6&partnerID=40&md5=5772ed7e12f54028d8703c5a7585e40e |
id |
2-s2.0-58449128324 |
---|---|
spelling |
2-s2.0-58449128324 Rosli N.; Maziah M.; Chan K.L.; Sreeramanan S. Factors affecting the accumulation of 9-methoxycanthin-6-one in callus cultures of Eurycoma longifolia 2009 Journal of Forestry Research 20 1 10.1007/s11676-009-0010-6 https://www.scopus.com/inward/record.uri?eid=2-s2.0-58449128324&doi=10.1007%2fs11676-009-0010-6&partnerID=40&md5=5772ed7e12f54028d8703c5a7585e40e A study was conducted to improve 9-methoxycanthin-6-one productivity (potential anti-tumour compound) from callus cultures of Eurycoma longifolia (Tongkat Ali). Several factors affecting 9-methoxycanthin-6-one production in callus cultures such as different medium compositions and physical factors were investigated and analyzed. Results show that a higher production of 9-methoxycanthin-6-one (3.84 mg•g-1 DW (Dry Weight)) is obtained from callus cultured in 3/4 MS basal media. At fructose of 2% (w/v), the production of 9-methoxycanthin-6-one (4.59 mg•g-1 DW) is promoted to gain the highest yield, compared to other carbon sources tested. The addition of 2.0-mg•L-1 dicamba also increases 9-methoxycanthin-6-one production (12.3 mg•g-1 DW). Higher production of 9-methoxycanthin-6-one was obtained at pH 5.5 (1.53 mg•g -1 DW). Production of 9-methoxycanthin-6-one (2.34 mg•g -1 DW) in callus cultures is also increased when the medium is added with 1×10-1 μM phenylalanine. This study suggests that the successful production of 9-methoxycanthin-6-one in vitro cultures has a potential in large-scale production using bioreactor technology. © 2009 Northeast Forestry University and Springer-Verlag GmbH. Springer Nature 1007662X English Article |
author |
Rosli N.; Maziah M.; Chan K.L.; Sreeramanan S. |
spellingShingle |
Rosli N.; Maziah M.; Chan K.L.; Sreeramanan S. Factors affecting the accumulation of 9-methoxycanthin-6-one in callus cultures of Eurycoma longifolia |
author_facet |
Rosli N.; Maziah M.; Chan K.L.; Sreeramanan S. |
author_sort |
Rosli N.; Maziah M.; Chan K.L.; Sreeramanan S. |
title |
Factors affecting the accumulation of 9-methoxycanthin-6-one in callus cultures of Eurycoma longifolia |
title_short |
Factors affecting the accumulation of 9-methoxycanthin-6-one in callus cultures of Eurycoma longifolia |
title_full |
Factors affecting the accumulation of 9-methoxycanthin-6-one in callus cultures of Eurycoma longifolia |
title_fullStr |
Factors affecting the accumulation of 9-methoxycanthin-6-one in callus cultures of Eurycoma longifolia |
title_full_unstemmed |
Factors affecting the accumulation of 9-methoxycanthin-6-one in callus cultures of Eurycoma longifolia |
title_sort |
Factors affecting the accumulation of 9-methoxycanthin-6-one in callus cultures of Eurycoma longifolia |
publishDate |
2009 |
container_title |
Journal of Forestry Research |
container_volume |
20 |
container_issue |
1 |
doi_str_mv |
10.1007/s11676-009-0010-6 |
url |
https://www.scopus.com/inward/record.uri?eid=2-s2.0-58449128324&doi=10.1007%2fs11676-009-0010-6&partnerID=40&md5=5772ed7e12f54028d8703c5a7585e40e |
description |
A study was conducted to improve 9-methoxycanthin-6-one productivity (potential anti-tumour compound) from callus cultures of Eurycoma longifolia (Tongkat Ali). Several factors affecting 9-methoxycanthin-6-one production in callus cultures such as different medium compositions and physical factors were investigated and analyzed. Results show that a higher production of 9-methoxycanthin-6-one (3.84 mg•g-1 DW (Dry Weight)) is obtained from callus cultured in 3/4 MS basal media. At fructose of 2% (w/v), the production of 9-methoxycanthin-6-one (4.59 mg•g-1 DW) is promoted to gain the highest yield, compared to other carbon sources tested. The addition of 2.0-mg•L-1 dicamba also increases 9-methoxycanthin-6-one production (12.3 mg•g-1 DW). Higher production of 9-methoxycanthin-6-one was obtained at pH 5.5 (1.53 mg•g -1 DW). Production of 9-methoxycanthin-6-one (2.34 mg•g -1 DW) in callus cultures is also increased when the medium is added with 1×10-1 μM phenylalanine. This study suggests that the successful production of 9-methoxycanthin-6-one in vitro cultures has a potential in large-scale production using bioreactor technology. © 2009 Northeast Forestry University and Springer-Verlag GmbH. |
publisher |
Springer Nature |
issn |
1007662X |
language |
English |
format |
Article |
accesstype |
|
record_format |
scopus |
collection |
Scopus |
_version_ |
1809678489260916736 |